正交实验优化异甘草素超声提取工艺  

Optimization ultrasonic extraction process of isoliquiritigenin by orthogonal method

作　　者：付玉杰[1] 刘晓娜[1] 施晓光[1] 李双明[1] 祖元刚[1] 赵春建[1] 

机构地区：[1]东北林业大学教育部森林植物生态学重点实验室,黑龙江哈尔滨150040

出　　处：《化学工程》2007年第1期75-78,共4页Chemical Engineering(China)

基　　金：教育部新世纪优秀人才支持计划资助(NCET-04-0316);黑龙江省重点自然基金项目(ZJN04-03);黑龙江省留学回国人员科技活动择优资助经费(黑人函[2005]311号);黑龙江省博士后启动金资助

摘　　要：采用超声法提取乌拉尔甘草中异甘草素,通过乙醇体积分数、液固质量体积比(g/mL)、超声提取时间和提取次数4个因素对异甘草素提取率的影响进行了正交实验,确定超声提取最佳工艺条件为:体积分数80%乙醇,液固质量体积比(g/mL)10∶1,超声20 m in,提取3次,异甘草素的提取率为0.37‰,是乙醇热回流提取的2.06倍,是索氏提取的1.54倍。超声提取浸膏中异甘草素的质量分数为0.55%,是乙醇热回流提取的3.24倍,是索氏提取的1.45倍。结果表明:超声提取甘草中异甘草素具有提取温度低、速度快、提取率高、纯度高等特点。Ultrasonic extraction method was used to extract isoliquiritigenin （ISLG） from Glycyrrhiza uralensis Fisch. The extraction conditions that influenced the extraction yield of ISLG were studied, including volume fraction of alcohol, ratio of liquid to material （g/mL）, ultrasonic-irradiation time and extraction times. The results of orthogonal test indicated the optimum ultrasonic extraction conditions of ISLG： volume fraction 80% alcohol as solvent, ratio of liquid to material 10：1 （g/mL）, ultrasonic extraction time 20 min, 3 times for extraction. The yield of ISLG was 0.37%o, which was 2.06 times as high as those of heat reflux extraction, 1.54 times of Soxhlet extraction. The mass fraction of ISLG in the crude extracts was 0.55%, which was 3.24 times of heat reflux extraction, 1.45 times of Soxhlet extraction. The results show that ultrasonic extraction is efficient, simple and rapid for extracting ISLG from Glycyrrhiza uralensis Fisch.

关 键 词：正交实验 超声提取 乌拉尔甘草 异甘草素 

分 类 号：TQ461[化学工程—制药化工]