咳喘宁对支气管哮喘大鼠肺组织一氧化氮和内皮素-1含量的影响  

Effects of Kechuanning on NO and ET-1 in Lung Tissue of Rat with Bronchial Asthma

作　　者：王晓红[1] 杨牧祥[2] 于文涛[2] 徐华洲[2] 段旭东[1] 

机构地区：[1]河北医科大学第二医院,石家庄050000 [2]河北医科大学中医学院,石家庄050091

出　　处：《中华中医药杂志》2007年第1期12-15,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：河北省科技攻关计划项目(No.06276102D-37)

摘　　要：目的:观察咳喘宁对支气管哮喘大鼠肺组织一氧化氮(NO)和内皮素(ET-1)含量的影响。方法:40只SD大鼠随机分为正常组、模型组、咳喘宁高剂量组(27g生药/kg体质量)、咳喘宁低剂量组(13.5g生药/kg体质量)、桂龙咳喘宁组(0.41g/kg体质量),每组8只。除正常组外以卵蛋白致敏并吸入激发法制备大鼠支气管哮喘模型,各治疗组均从第1次哮喘激发开始(造模第3周)至处死前每天灌胃给药,激发并给药4周后处死大鼠,观察肺组织NO、ET-1含量和病理组织学变化。结果:与正常组比较,模型组大鼠支气管管壁和平滑肌厚度、嗜酸性粒细胞、淋巴细胞数以及肺组织NO、ET-1含量明显增加(P<0.01);与模型组比较,各治疗组均可显著降低支气管管壁和平滑肌厚度、嗜酸性粒细胞、淋巴细胞数以及肺组织NO、ET-1含量(P<0.05或P<0.01);且咳喘宁高、低剂量组优于桂龙咳喘宁胶囊组(P<0.05或P<0.01)。结论:咳喘宁可通过降低肺组织NO和ET-1含量,抑制支气管哮喘大鼠气道炎症和气道重塑等病理过程。Objective： To observe the effects of Kechuanning on nitrogen monoxidum （NO） and endothelin （ET-1） of in pulmonary tissue of rat with bronchial asthma. Methods： 40 rats were randomly divided into 5 groups： the normal group, the model group, Kechuanning high-dose group （27g/kg）, Kechuanning low-dose group （13.5g/kg） and Guilong Kechuanning Capsule control group （0.41g/kg）, 8 rats each group. The bronchial asthmatic model was established by egg protein sensibilization and long-term inhalation provocation. The rats of each treatment groups were given intragastric administration each day from the first time of provocation to execution. After 4 weeks of treatment,the rats were killed and lung tissue were taken to dyeing of HE to be observed. The contents of NO and ET-1 in lung tissue were determined. Results： Compared with the normal group, the thickness of bronchus wall and bronchus smooth muscle, the numbers of eosinophile granulocyte and leukomonocyte, and the contents of NO and El＇- 1 in lung tissue were increased in the model group （ P 〈 0.01） ; Com- pared with the model group, the thickness of bronchus wall and bronchus smooth muscle, the numbers of eosinophile granu- locyte and leukomonocyte and the contents of NO and ET-1 in lung tissue were decreased in the each treatment group （ P 〈 0.05 or P 〈 0.01）; And Kechuanning high-dose and low-dose groups surpass Guilong Kechuanning Capsule group（ P 〈 0.05 or P 〈 0.01）. Conclusion： Kechuanning could decrease the contents of NO and ET- 1 in lung tissue, so as to restrain air passage inflammation and against airway remolding.

关 键 词：咳喘宁 支气管哮喘 一氧化氮 内皮素 

分 类 号：R285.5[医药卫生—中药学]