RP-HPLC测定木通药材中皂苷类成分的含量  

Determination of Triterpenoid Saponins in Caulis akebiae by RP-HPLC

作　　者：高慧敏[1] 王智民[1] 付雪涛[1] 王维皓[1] 王冠[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700

出　　处：《中国药学杂志》2007年第1期20-23,共4页Chinese Pharmaceutical Journal

基　　金："863"创新药物和中药现代化项目(2004AA223730-14)

摘　　要：目的 建立木通药材中2种皂苷类成分同时用HPLC测定的方法。方法 采用Phenomenex Luna C18（4．6min×250min，5μm）柱，以甲醇-乙腈-水-磷酸（20：20：60：0.1）为流动相，流速1．0mL·min^-1，检测波长为203nm，柱温35℃。结果 saponin PJ1和mutongsaponin C分别在Q25～4．0μg,0．21-3．4μg内呈良好的线性关系，平均加样回收率分别为98．11％和101．19％。对3个基源（三叶、五叶、白木通）的木通药材共30个样品进行了含量测定，并比较了两者在原植物根、茎、叶、果实（共10个样品）中的分布。结论 白木通和三叶木通中均含有2种被测成分，五叶木通大部分样品未检出。OBJECTIVE To establish a HPLC method for the determination of saponin PJ1 and mutongsaponin C in Caulis akebiae. METHODS The samples were separated at 35 ℃ using a Phenomenex lama C18 column with methanol-acetonitrile-water-phosphofic acid （20：20：60：0.1） as mobile phase. Flow rate was at 1. 0mL.min^-1 and the detection wavelength was at 203nm.RESULTS The calibration curves of saponin PJ1 and mutongsaponin C were linear over the ranges of 0. 25 -4. 0 μg, 0. 21 - 3. 4 μg and the average recoveries were 98. 1% and 101.2%, respectively. Forty crude materials from the root,stem,leaf and fruit, collected from different areas, were determined. CONCLUSION Saponin PJ1 and mutongsaponin C can be regarded as marker compounds for distinguishing Akebht trifoliata, A. trifoliata var. australis and A. quinata.

关 键 词：木通 皂苷PJ1 木通皂苷C 高效液相色谱法 

分 类 号：R284[医药卫生—中药学]