叶酸和聚乙二醇接枝作基因载体用壳聚糖的合成与表征  

Grafting chitosan with folic acid and poly(ethylene glycol)

作　　者：徐海娥[1] 闫翠娥[1] 

机构地区：[1]湖北大学化学化工学院,湖北武汉430062

出　　处：《化学研究与应用》2007年第1期60-63,共4页Chemical Research and Application

基　　金：武汉市青年科技晨光计划(020-093403)资助项目

摘　　要：本研究将叶酸和聚乙二醇接枝到四种不同分子量的壳聚糖氨基侧链上,以改善壳聚糖的靶向性和水溶性作基因载体。用FTIE、1HNMR、UV-Vis、DSC和TEM对产物进行了表征,结果表明,叶酸和聚乙二醇被成功地接枝到壳聚糖上,所制得的载体有望作为潜在的肿瘤细胞靶向基因载体。Chitosan is one of best non - viral gene carriers. Folio acid can specifically interact with folate receptor located on the plasma cell membrane. Poly（ ethylene glycol） can improve the solubility of chitosan and prolong plasma circulation time of chitosan/DNA complexes. In this study, folio acid and methoxy - polyethylene glycol acetic acid were grafted onto chitosan with different molecular weigh through their primary amino groups for target gene delivery. The successful grafting were proved by FTIR, ^1 H NMR, UV- Vis and TEM.

关 键 词：壳聚糖 叶酸 聚乙二醇 接枝 

分 类 号：O629.4[理学—有机化学] O633.11[理学—化学]