NBS-荧光素-盐酸头孢吡肟化学发光体系的研究  

Flow injection chemiluminescence method for the determination of cefepime hydrochloride based on NBS-fluorescein reaction

作　　者：张淑琼[1] 

机构地区：[1]涪陵师范学院化学及环境科学系,重庆408003

出　　处：《化学研究与应用》2007年第1期118-120,共3页Chemical Research and Application

基　　金：重庆市教委科学研究项目(041304)资助

摘　　要：A flow injection chemiluminescence method for the determination of Cefepime hydrochloride is descrbed.The method is based on the chemiluminesence emitted during the oxidation of Cefepime hydrochloride by N-bromosuccinimide（NBS）in alkaline medium in the presence of fluorescein as energy transfer.The chemiluminescence intensity is proportional to cefepime hydrochloride concentration over the range 0.05mg·L-110mg·L-1.The detection limit is 29μg.L-1and the relative standard deviation is 4.3% for 5mg·L-1cefepime hydrochloride（n=11）.This method had been successfully applied to determination of cefepime hydrochloride in injections.A flow injection chemiluminescence method for the determination of Cefepime hydrochloride is descrbed. The method is based on the chemiluminesence emitted during the oxidation of Cefepime hydrochloride by N- bromosuccinimide （NBS）in alkaline medium in the presence of fluorescein as energy transfer. The chemiluminescence intensity is proportional to cefepime hydrochloride concentration over the range 0. 05mg · L^-l ～ 10mg ·L^-l. The detection limit is 29μg. L^-l and the relative standard deviation is 4. 3% for 5mg· L^-lcefepime hydrochloride（n = 11 ）. This method had been successfully applied to determination of cefepime hydrochloride in injections.

关 键 词：化学发光 流动注射 盐酸头孢吡肟 N-溴代琥珀酰亚胺 荧光素 

分 类 号：O657.39[理学—分析化学]