双6-氧■3-间硝基苯磺酰基-丁二酸-1,4-单酯-4■β-环糊精的合成及模拟抗坏血酸氧化酶  

Synthesis of Bis6-Oxygen■3-m-Nitrobenzene Sulfonyl-Succinic Acid-1,4 Monoester-4■β-CD and Mimic Ascorbic Oxidase

作　　者：杨曦[1] 沈静茹[1] 李云[1] 

机构地区：[1]中南民族大学化学与材料科学学院,武汉430074

出　　处：《中南民族大学学报（自然科学版）》2006年第4期28-30,共3页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：湖北省自然科学基金资助项目(95J60)

摘　　要：为建立更多更好的模拟酶定量测定方法,用β-环糊精、马来酸酐、间硝基苯磺酰氯和三氯化铁反应,合成了双6-氧3-间硝基苯磺酰基-丁二酸-1,4-单酯-4β-环糊精.F e3+配合物,以此配合物与H2O2形成复合物,模拟抗坏血酸氧化酶,将抗坏血酸催化脱氢生成脱氢抗坏血酸.在最佳条件下,催化反应可在3m in内反应完全.初速度显示出良好的线性,模拟酶催化速率较快,为下一步用此模拟酶测定相关物质含量提供了可能.In order to set up a better method for detecting substract with a mimic enzyme, a new β-cyclodextrin derivative was synthesized from β-cyclodextrin, maleic anhydride and m-nitrobenzene sulfonyl chloride. A new mimic aseorbie acid oxydase can be constructed by the β-cyclodextrin derivative, iron trichloride with hydrogen peroxide solution. In the best condition, the substance can be completely e atalysed by this mimic enzyme within 3 min. The initial velocity is better linear to the concentration. The mimic enzyme can be more effective in reaction and provides posibility for detecting content of substraet next step.

关 键 词：Β-环糊精衍生物 合成 模拟抗坏血酸氧化酶 

分 类 号：O623.624[理学—有机化学] Q811.2[理学—化学]