羊栖菜多糖分离纯化和结构的初步鉴定  

Isolation,purification and determination of structure of polysaccharides from sargassum fusiforme

作　　者：汲晨锋[1] 吴涛[2] 王翀[2] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研发中心,黑龙江哈尔滨150076 [2]哈尔滨商业大学药物研究所博士后科研工作站,黑龙江哈尔滨150076

出　　处：《哈尔滨商业大学学报（自然科学版）》2006年第6期8-12,共5页Journal of Harbin University of Commerce：Natural Sciences Edition

摘　　要：将提取的羊栖菜多糖用sevage法除蛋白、H2O2脱色后得到羊栖菜多糖半纯品,依次通过DE-AE-52、Sephadex G-200柱层析分离纯化后得到SFPS-B2、SFPS-C1、SFPS-D2三个多糖组分,用Sephacryl S200-HR柱层析鉴定纯度,三种组分的多糖为均一多糖,质量分数分别为86.02%、81.05%、86.95%.对SFPS-D2进行结构初步研究,通过紫外光谱、红外光谱和毛细管电泳分析,结果表明SFPS-D2不含蛋白质和核酸,多糖组成以β构型的吡喃糖苷键为主,含有硫酸基-O-SO3,其单糖组成以木糖、葡萄糖和半乳糖为主,其比例为木糖∶葡萄糖∶半乳糖=3.45∶5.8∶1.To obtain semi-purified polysaccharides from sargassum fusiforme by sevage＇s method and H2O2. The semi-purified polysaccharides is further purified by loading on DEAE - 52 and Sephadex G - 200 respectively. Three groups （SPFS - B2, SPFS - C1, SPFS - D2） is obtained and proved to be homogeneous polysaccharides by Sehacryl S200 - HR. The determination of polysaccharides was 86.02%, 81.05%, 86.95%. The SFPS - D2 primary structure was studied by UV, IR and HPCE. The results showed that the SFPS - D2 was mainly linked by β - glycosidics in its back bone and mainly composed of xylose, glucose, galactose （the ratio is 3.45 ： 5.8 ： 1） and sulfate （-O-SO3） protein and nucleic acid is not found in SPFS - D2.

关 键 词：羊栖菜 多糖 分离纯化 结构鉴定 毛细管电泳 

分 类 号：R289[医药卫生—方剂学]