反相高效液相色谱法测定马钱子不同炮制品中士的宁与马钱子碱的含量  

RP-HPLC Determination of Strychnine and Brucinein from Semen Strychni and Its Processed Products

作　　者：周道根[1] 张的凤[1] 龚千峰[1] 陈泣[1] 

机构地区：[1]江西中医学院,江西南昌330004

出　　处：《时珍国医国药》2007年第1期33-34,共2页Lishizhen Medicine and Materia Medica Research

基　　金：江西省科技攻关资助项目(赣科发计字[2003]245)

摘　　要：目的用反相高效液相色谱(RP-HPLC)法对马钱子生品及炮制品中士的宁和马钱子碱含量进行测定,探讨醋制马钱子炮制原理。方法Hypersil BDS C18(4.6 mm×200 mm,5μm),流动相为乙腈-水-二乙胺(34∶100∶1),检测波长260nm。结果江西改良炮制法炮制品中士的宁和马钱子碱的含量较低。结论该法精确、简便、重现性好。Objective To develop an RP-HPLC method for determination of strychnine and brucine in Semen Strychni and its processed products and study the principle of Semen Strychni processed products with Jiangxi innovated method. Methods A Hypersil BDS C18 （4.6 mm ×200 mm, 5 μm） column was used with acetonitrile-water-diethylamine（34： 100： 1 ） as the mobile phase , the content was detected at a wavelength of 260 nm. Results The contents of strychnine and brucine in the processed products with Jinngxi innovated method were lower. Conclusion This method is accurate, simple and reliable.

关 键 词：马钱子 炮制 士的宁 马钱子碱 反相高效液相色谱 

分 类 号：R284.2[医药卫生—中药学]