固相萃取-高效液相色谱法测定黄芪中黄芪甲苷的含量  

Determination of Astragaloside IV in Radix Astragali by SPE-HPLC

作　　者：杨克迪[1] 李宏[1] 龙云飞[1] 郑智慧[1] 万顺刚[1] 

机构地区：[1]广西大学化学化工学院,广西南宁530004

出　　处：《时珍国医国药》2007年第1期41-42,共2页Lishizhen Medicine and Materia Medica Research

基　　金：广西教育厅资助项目[项目批准号:桂教科研(2004)20]

摘　　要：目的采用固相萃取-高效液相色谱方法分析黄芪中黄芪甲苷的含量。方法采用C18固相萃取小柱纯化黄芪提取物中的黄芪甲苷，反相高效液相色谱-紫外检测方法测定黄芪中黄芪甲苷含量。色谱柱为Zorbax SB-C18柱（φ4．6mm×150mm，5μm），以乙腈-水（32：68）为流动相．流速1．0ml／min，柱温30℃，检测波长200nm。结果黄芪甲苷进样量在1.408～7．04μg范围内与峰面积呈良好线性关系，r=0．9999。平均回收率为101．5％。结论该方法操作简便。重现性好，可作为黄芪药材或其它含黄芪制剂中黄芪甲苷含量的分析方法。Objective To establish SPE-HPLC method for the determination of astragaloside Ⅳ in Radix Astragali. Methods The astragaloside Ⅳin sample was purified with C18 solid phase column and its content was determined with HPLC-UV method. The astragaloside Ⅳ was separated on a Zorbax SB-C18 column （φ4.6 mm× 150 mm, 5 μm） with acetonitrilewater （32： 68, v/v） as mobile phase, and the detective wavelength was set at 200nm. Results The linear range was 1. 408 - 7.04 μg （ r = 0.999 9）. The average recovery of astragaloside Ⅳ was 101.5%. Conclusion The method is simple, accurate and can be used for the quality control of Radix Astragali and its preparation.

关 键 词：固相萃取 高效液相色谱 黄芪甲苷 

分 类 号：R284.2[医药卫生—中药学]