电针对大鼠脑缺血再灌注模型P-选择素和E-选择素表达影响的实验研究  

Effect of Galvano-acupuncture on the Expression of P-selectin and E-selectin of Cerebral Ischenic Region in Rats

作　　者：刘玉珍[1] 韩景献[2] 蒋戈利[1] 丁晓荣[2] 陆明霞[2] 

机构地区：[1]北京军区天津疗养院中医针灸康复中心,天津300381 [2]天津中医药大学第一附属医院老年病研究室,天津300193

出　　处：《时珍国医国药》2007年第1期79-81,共3页Lishizhen Medicine and Materia Medica Research

摘　　要：目的探讨针刺抗脑缺血再灌注炎性损伤的机理。方法采用线栓法制作大鼠大脑中动脉缺血模型(MCAO),采用免疫组织化学技术测定P-选择素和E-选择素表达的动态变化。结果在灌注3 h各实验组P-选择素和E-选择素均开始表达,二者均于再灌注24 h均达到高峰(组内比较P<0.01),持续至再灌注48 h,针刺可显著降低P-选择素和E-选择素的表达(与模型组和非穴组比较,P<0.01)。结论P-选择素和E-选择素的表达上调与再灌注后的炎症反应有关,早期针刺治疗可能通过下调脑缺血区黏附分子P-选择素和E-选择素的表达而防治脑缺血再灌注炎性损伤。Objective To study mechanisms of early treatment of cerebral ischemia- reperfusion（I/R） by galvano- acupuncture in resisting inflammation injury in rats. Methods Cerebral I./R model was established by occlusion of the middle cerebral artery for lh and then removal of the occlusion for 3 h ,12 h,24 h, and 48 h respectively. The expression of P - selectin and E-selectin was displayed by using imunohistochemical ABC in ischemic cerebral cortex and corpus striatum in rats. Results The expression of P - selectin and E-selectin was detected at capillary walls in postischemic cerbral cortex at 1 h ischemia - 3 h reperfusion and peaked at 1 h ischemia - 24 h reperfusion and increased continuously during 48 h reperfusion. The expression of both P-selectin and Eselectin in ischemic cerebral cortex were down-regulated considerably by galvano - acupuncture treatment （ P 〈 0.01 ） . Conclusion The protective effect of acupuncture on cerebral neurons under acute infarction is possible through its downregulative action on the expression of P-selectin and E-selectin, which causes neutrophil infiltration that induced by adhesive molecules between endothelium cell and neutrophil.

关 键 词：针刺 脑缺血/再灌注 P-选择素 E-选择素 

分 类 号：R285.5[医药卫生—中药学]