高效液相色谱法测定双黄连粉针剂中绿原酸和黄芩苷的含量  

Quantitative Determination of Chlorogenic Acid and Baicalin in Shuanghuanglian Powder Injection by HPLC

作　　者：杨瑞芬[1] 彭家钢[1] 周蓉[1] 达世禄[2] 杨志[3] 

机构地区：[1]湖北中医学院,湖北武汉430065 [2]武汉大学化学与分子科学学院,湖北武汉430072 [3]华中科技大学同济医学院,湖北武汉430030

出　　处：《时珍国医国药》2007年第1期124-126,共3页Lishizhen Medicine and Materia Medica Research

摘　　要：目的建立高效液相色谱（HPLC）法同时测定双黄连粉针剂中绿原酸和黄芩苷外标定量测定方法。方法用自制C8～C13混合型烷基键合硅胶柱（5μm，4．6mm×150mm）为固定相，甲醇和水溶液（用磷酸调pH2．7）为流动相，在C8-C13柱上梯度洗脱，流速为1ml·min^-1，紫外检测波长为323nm。结果绿原酸和黄芩苷的线性范围分别为4．0-100．0mg·ml^-1和8．6—215．0mg·ml^-；相关系数分别为0．99989和0．99997；加样回收率分别为100．53％和99．94％；检测限分别为0．014mg·ml^-1和0．020mg·ml^-1；精密度实验RSD分别为1．12％和0．61％；重现性实验RSD分别为1．14％和0．73％，稳定性实验RSD分别为0．89％和0．54％；4批样品中绿原酸的含量在1．577～1．753mg·ml^-1，黄芩苷的含量在26．02-28．68mg·ml^-1。结论该方法简便，快速，准确，灵敏虚离。重现性好，成本低，可用于双黄连粉针剂的质量控制。Objective To develop an RP - HPLC method for the determination of chlorogenic acid and baicalin in Shuanghuanglian powder injection. Methods A C8- C13 mixedalkyl （51μm,4.6 mm × 150 mm） bond silica for reversed - phase HPLC was prepared. The mobile phase was CH3OH - phosphoric acid solution （ pH 2.7）. The flow rate was set at 1. 0 ml/min. The detection wavelength was at 323 nm. Chlorogenic acid and baicalin were separated by HPLC with grade elution. Results The linearity ranges of chlorogenic acid and baicalin were 4.0 - 100.0 mg/ml and 8.6 - 215.0 mg/ml respectively, the correlation coefficients were 0.999 89 and 0.999 97 ,the average recoveries of adding sample were 100.53% and 99.94%, detection limits were 0. 014 mg/ml and 0. 020 mg/ml, the RSD （n = 5） of measurement precision test were 1. 12% and 0.61%, the RSD （ n = 5） of reproducibility between tests were 0.89% and 0.54%. The content of chlorogenic acid in Shuanghuanglian powder was 1. 577 - 1. 753 mg/ml,and that of baicalin was 26.02 - 28.68 mg/ml. Conclusion The method is simple, fast, accurate,sensitive, reproducible and low cost. It is fit for the quality control of Shuanghuanglian powder.

关 键 词：双黄连粉针剂 绿原酸 黄芩苷 高效液相色谱法 梯度洗脱 

分 类 号：R284.2[医药卫生—中药学]