丙酸钠和丙酮酸钠对体外培养牛肝细胞内丙酮酸羟化酶基因mRNA水平的影响  

Effects of propionate sodium and pyruvate sodium on mRNA level of PC gene in cultured bovine hepatocytes in vitro

作　　者：徐闯[1] 郝贵增[2] 刘国文[1] 王哲[1] 夏成[3] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]安阳工学院生物与食品学院,河南安阳455000 [3]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319

出　　处：《中国兽医科学》2007年第1期78-82,共5页Chinese Veterinary Science

基　　金：国家自然科学基金重点项目(30230620)

摘　　要：在成功构建牛肝细胞培养模型和PC基因DNA竞争模板的基础上,采用竞争RT-PCR方法检测了丙酸钠和丙酮酸钠对体外培养新生牛单层肝细胞PC基因mRNA丰度的影响。结果,随着丙酸钠浓度的升高,PC基因mRNA水平呈上升趋势,PC基因mRNA对丙酸钠的耐受范围较广;随着丙酮酸钠浓度的升高PC基因mRNA水平呈先上升后下降的趋势。结果表明,肝细胞内PC基因mRNA的表达水平受丙酮酸钠浓度的调控,在一定范围内丙酮酸钠对PC基因mRNA的转录具有促进作用,而浓度过高时则起抑制作用。On the successful basis of bovine hepatic cell culture and competitive DNA template of pyruvate carboxylase（PC） gene,effects of propionate sodium and pyruvate sodium on mRNA of PC gene in cultured bovine hepatocyte in vitro was determinated quantitively by competitive RT-PCR. Results showed that mRNA level of PC gene was enhanced with the increase of concentrations of propionate sodium,and tolerant range of PC mRNA to propionate sodium was wide. mRNA level of PC gene was enhanced when pyruvate sodium＇s concentration was low and decreased when its concentration was excessively high. The results showed that propionate sodium could regulate mRNA level of PC gene in bovine hepatocytes cultured in vitro, and pyruvate sodium could regulate PC gene transcription,but it depended on the concentrations of pyruvate sodium.

关 键 词：MRNA水平 丙酮酸钠 酶基因 体外培养 丙酸钠 细胞内 牛肝 PCR方法 

分 类 号：Q-33[生物学]