柞蚕核型多角体病毒PstI-B、C片段克隆及序列分析(英文)  

Clone and Sequence Analysis of Antheraea pernyi Nucleopolyhedrovirus PstI-B and C fragments

作　　者：石生林[1] 潘敏慧[1] 鲁成[1] 

机构地区：[1]西南大学农业部蚕桑学重点实验室

出　　处：《中国生物工程杂志》2007年第1期81-85,共5页China Biotechnology

基　　金：国家"973"计划资助项目(2005CB121000)~~

摘　　要：克隆并测序分析了柞蚕核型多角体病毒(Antheraea pernyinucleopolyhedrovirus,ApNPV)PstI-B和C片段。结果表明:ApNPVPstI-B片段长7406bp,编码7个开放阅读框(open readingframes,orf),包括p87、he65、pnk/pnl、odv-ec43基因及黄杉毒蛾核型多角体病毒(Orgyiapseudotsugatamulticapsid nucleopolyhedrovirus,OpMNPV)orf107、orf108同源区。ApNPVPstⅠ-C长6663bp,编码11个orf,包括pk-1、orf1629、polh、lef-2、ptp-2、ctl-1、ptp-1基因及OpMNPVorf5、orf7、orf8和orf11同源区。在鉴定的18个杆状病毒基因中,he65和orf1629基因分化较大;polh和lef-2基因较保守。ApNPV是已知的第3个编码pnk/pnl基因、第4个同时编码ptp-1和ptp-2两个基因的杆状病毒。Antheraea pernyi nucleopolyhedrovirus （ApNPV） PstI-B and C fragments were cloned and sequenced. ApNPV PstI-B was 7406 bp long, contained seven open reading frames （orfs）/genes, including p87, he65, pnk/pnl , odv-ec43 and Orgyia pseudotsugata muhicapsid nucleopolyhedrovirus （OpMNPV） orf107 , orf108 homologue on either strands of genomic DNA. ApNPV PstI-C was 6663 bp long, contained eleven orfs/genes, including pk-1, orf1629, polh, lef-2, ptp-2, ctl-1, ptp-1 and OpMNPV orfS, orf7, orf8, orf11 homologue on either strands of genomic DNA. Among the eighteen baculovirus genes identification, he65 and orf1629 were two diverse genes, while polh and lef-2 were two conserved genes. ApNPV was the third baculovirus found to contain pnk/pnl gene, the fourth baculovirus found to contain both ptp-1 and ptp-2 gene.

关 键 词：柞蚕 核型多角体病毒 杆状病毒 序列分析 

分 类 号：Q93[生物学—微生物学]