大鼠MSCs诱导分化为心肌样细胞时离子通道基因的表达  

Expression of ion channel gene during MSCs differentiating into cardiomyocytes

作　　者：王金菊[1] 田杰[1] 吕铁伟[1] 张小飞[1] 朱静[2] 李扬[1] 陈沅[1] 

机构地区：[1]重庆医科大学儿童医院心血管内科 [2]重庆医科大学儿科研究所,重庆400014

出　　处：《基础医学与临床》2007年第1期36-39,共4页Basic and Clinical Medicine

基　　金：国家自然科学基金(30271381)

摘　　要：目的研究大鼠骨髓间充质干细胞(MSCs)体外向心肌样细胞分化过程中L-型钙通道(α1C)和瞬间外向钾通道(kv4.3)基因的表达。方法无菌条件下冲洗Wistar大鼠双侧股骨和胫骨的骨髓腔获得MSCs,体外培养传代纯化,5-氮杂胞苷(5-aza)诱导24 h,显微镜下观察诱导前后形态变化,RT-PCR鉴定α1C和kv4.3基因的表达。结果kv4.3基因诱导前有弱的表达,诱导后1、4、7和14 d表达明显逐渐增强(P<0.05);α1C基因诱导前有较强的表达,诱导后1、4、7和14 d表达却明显逐渐减弱(P<0.05)。结论α1C和kv4.3基因在维持MSCs的诱导分化过程中发挥重要作用。Objective To study the expression of L-type calcium channel gene （α1c） and transient outward potassium channel gene（ kv4.3） during the procession of MSCs differentiating into eardiomyoeytes. Methods MSCs were isolated cultured, passaged, purified, and then exposed to 5-aza for 24 hours. The expression of α1C and kv4. 3 gene was detected by reverse transeription-polymerase chain reaction.Result MSCs had already expressed α1c and kv4. 3 mRNA before 5-aza treatment.The expression of kv4. 3 was significantly increased after 5-aza exposure at 24 hoursand at 4th,7th day and 14th day,wheras the expression of α1C was impaired. Conlusion L-type calcium channel and transient outward potassium channel played important roles during procession of MSCs differen-flaring into cardiomyocytes.

关 键 词：骨髓间充质干细胞 诱导 5-氮杂胞苷 离子通道 

分 类 号：Q343[生物学—遗传学] Q813