脂多糖诱导骨关节炎滑膜细胞表达一氧化氮与氨基胍的抑制效应  

Inhibitive effect of aminoguanidine on the expression of nitric oxide in osteoarthritic synoviocytes induced by lipopolysaccharide

作　　者：刘传芳[1] 江建明[1] 梁鹏展[1] 孙炜[2] 

机构地区：[1]南方医科大学南方医院脊柱骨病科,广东省广州市510515 [2]深圳市第二人民医院脊柱外科,广东省深圳市518035

出　　处：《中国组织工程研究与临床康复》2007年第4期689-691,I0002,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金(30571890)~~

摘　　要：目的:观察氨基胍对脂多糖诱导骨关节炎滑膜细胞表达一氧化氮的影响,进一步了解炎性因子与一氧化氮之间的相互关系。方法:实验于2006-3/2006-7在南方医科大学组织工程研究中心进行。①6例骨关节炎滑膜,体外培养滑膜细胞。②将细胞浓度为1×108L-1,培养3～5代的滑膜细胞,以0.5mL/孔的量,加入至24孔板中,培养24h后,分别加入0,1,10,100mg/L脂多糖,及20mg/L脂多糖分别加0,0.1,1,10mmol/L氨基胍,继续培养48h后,收集培养液上清液。测量不同浓度脂多糖诱导下培养上清液中诱导型一氧化氮合酶的含量变化和同一浓度脂多糖+不同浓度氨基胍培养上清液中一氧化氮的含量变化。结果:①未加入脂多糖组培养上清液中诱导型一氧化氮合酶的含量极低,与培养基对照组比较差异无显著性意义(P>0.05)。诱导型一氧化氮合酶的含量随加入脂多糖浓度的增加而逐渐增加,各组间比较,差异有显著性意义(P<0.05)。②滑膜细胞在20mg/L脂多糖诱导下,0,0.1,1,10mmol/L氨基胍组一氧化氮表达的量逐渐减少,组见比较差异有显著性意义(P<0.05)。结论:在炎症因子脂多糖的作用下,滑膜细胞诱导型一氧化氮合酶的含量明显增加,诱导型一氧化氮合酶抑制剂氨基胍可以减少一氧化氮的生成,有助于阻断炎性因子与一氧化氮相互促进的恶性循环。AIM： To investigate the effect of aminoguanidine （AG） on expression of nitric oxide （NO） in osteoarthritic synoviocytes induced by lipopolysaccharide （LPS）, understand further the relationship between inflammatory cytokines and NO. METHODS： The experiment was conducted at the Research Center of Tissue Engineering of the Southern Medical University between March 2006 and July 2006. ①The osteoarthritic synoviocytes were isolated from 6 human articular synovium and cultured in vitro. ②The 3-5 generations synoviocytes were adjusted into 1×10^8 L^-1, infused the 24 well cluster, at 0.5 mL/well, after 24-hour culture, infused LPS of different concentration （0,1,10,100 mg/L） and （or） 20 mg/L LPS and AG of different concentration （0,0.1,1,10 mmol/L）, continued culture for 48 hours, collected supematant. The concentration change of the inducible nitric oxide synthase （iNOS） in the supernatant of the synoviocytes under the induction of LPS of different concentration, and the NO in the supernatant of the synoviocyte under the induction of LPS of the same concentration and AG of different concentration were detected. RESULTS：①The content of iNOS was extremely low in the group without LPS, and there was no significant difference as compared with the medium group （P 〉 0.05）, The content of iNOS increased with the increase of LPS concentration. There were significant differences among the groups. ② The production of NO deceased following the increase of AG＇s concentration （0,0.1,1,10 mmol/L） under the induction of 20 mg/L LPS, and there were significant differences among groups （P 〈 0.05）. CONCLUSION： The iNOS expression by osteoarthritic synoviocytes boosts under LPS, AG can inhibit the activity of iNOS, decrease the production of NO, help to block the vicious cycle between NO and inflammatory cytokines.

关 键 词：一氧化氮 滑膜/药物作用 骨关节炎/药物疗法 一氧化氮合酶/拈抗剂和抑制剂 

分 类 号：R684[医药卫生—骨科学]