草地早熟禾植株再生体系建立的初步研究  

Preliminary Studies on Plant Regeneration System of Poa pratensis L.

作　　者：段碧华[1] 韩宝平[1] 高遐虹[1] 段建平[1] 

机构地区：[1]北京农学院植物科学技术系,北京102206

出　　处：《北京农学院学报》2006年第4期20-24,共5页Journal of Beijing University of Agriculture

基　　金：国家高新研究发展计划(863)项目(2002AA2Z4011);北京市科技新星资助项目(H020821300120)

摘　　要：为培育优良抗性品种,选取新歌来德(Nuglade)、自由Ⅲ(Freedom Ⅲ)和优异(Merit)3个草地早熟禾品种的不同外植体,在不同浓度的6-BA,KT和NAA的培养基上进行植株培养,研究其对愈伤组织诱导率、绿苗分化率及生根的影响。结果表明:在不同培养基上,不同外植体愈伤组织的诱导率有较大的差异。草地早熟禾种子的愈伤组织诱导和继代培养基以MS(有机)+B5(无机)+2 mg/L 2,4-D+0.4 mg/L 6-BA为最佳;分化培养基以MS(有机)+B5(无机)+1.0 mg/L KT或MS(有机)+B5(无机)+1.0 mg/L 6-BA为最佳;生根培养基以1/2(MS(有机)+B5(无机))+0.8 mg/L NAA最佳。初步建立起草地早熟禾的植株再生体系。The different explants of Poa pratensis L. cv. （Nuglade,Merit and Freedom Ⅲ） were cultivated on the different medium with 6-BA,KT and NAA. The effect of different medium on the frequency of callus induction,green plant, roots were studied. A preliminary plant regeneration system of Poa pratensis L. was established. The result showed that：the frequency of callus induction for three explants was different. The best medium for callus induction of seeds was MS（organic）＋BS（inorganic）＋2 mg/L 2,4-D＋0.4 mg/ L 6-BA,seedling differentiation was MS（organic） ＋B5 （inorganic） ＋0.9 mg/L KT. and rhizogenesis was 1/2 （MS（organic）＋BS（inorganic））＋0.8 mg/L NAA for Poa pratensis L.

关 键 词：草地早熟禾 外植体 植株再生体系 

分 类 号：S543.035.3[农业科学—作物学]