利用多种SSR引物构建小麦遗传连锁图谱及其多态性分析  

Construction of Wheat Molecular Linkage Map Using Different SSR Markers and the Polymorphism of the Markers

作　　者：李卫华[1] 刘伟[2] 尤明山[1] 许杰 刘春雷[1] 李保云[1] 刘广田[1] 

机构地区：[1]中国农业大学农业部作物基因组学与遗传改良重点开放实验室/北京市作物遗传改良重点实验室,北京100094 [2]石河子大学,新疆石河子832003

出　　处：《麦类作物学报》2007年第1期1-6,共6页Journal of Triticeae Crops

基　　金：国家自然科学基金项目(30471076);国家"863计划"项目(2003AA207080)

摘　　要：为完善小麦遗传图谱并对小麦主要品质性状进行QTL定位,以小麦京771和Pm97034及其173个后代重组自交系(RIL)为作图群体,利用906对SSR引物筛选出RIL群体双亲表现多态性的引物270对,多态性频率为29.8%。利用这270对引物对RIL群体进行分析,共检测到184个多态性标记位点,利用其中的129个标记构建小麦分子的遗传连锁图谱。用Mapmaker 3.0和Mapdraw V2.1软件将129个SSR位点绘在小麦遗传连锁图上,该图谱覆盖小麦基因组全长2 106.2 cM,标记间的平均遗传距离为16.32 cM。173 recombinant inbred lines （RILs） derived from Jing 771 X Pm97034 were used as mapping population in wheat. 906 pairs of SSR primers were used to detect the polymorphisms between parents Jing 771 and Pm97034. 270 pairs of SSR primers with the polymorphisms between parents Jing 771 and Pm97034 were selected to analyze RIL population. 184 markers were detected by the primers mentioned above. Only 129 markers were used to construct the molecular genetic linkage map, and mapped on 21 chromosomes by Mapmaker 3.0 and Mapdraw V2.1. The map spaned about 2106.2 cM in wheat genome. The average distance of markers was 16.32 cM.

关 键 词：小麦 重组自交系群体 SSR标记 连锁图谱 多态性 

分 类 号：S512.1[农业科学—作物学] S330