室内主要挥发性有机物对离体培养胚胎发育的影响  

Effect of main indoor volatile organic compounds on the development of cultured rat embryos in vitro

作　　者：张全新[1] 朱伟[1] 周颖[1] 袁立懋[1] 

机构地区：[1]广州市疾病预防控制中心,广东广州510080

出　　处：《中国职业医学》2007年第1期24-25,28,共3页China Occupational Medicine

基　　金：广州市医药卫生科技基金资助(2004-088)

摘　　要：目的探讨室内主要挥发性有机物对大鼠胚胎发育的影响。方法采用植入后全胚胎培养模型,将9.5dSD大鼠胚胎与吸入染毒的大鼠即刻离心血清共培养48h,观察空气染毒大鼠血清对离体培养胚胎生长发育和组织器官形态分化的影响。结果大鼠在模拟小室中染毒10d,染毒浓度约为现场检测最高值的2、4、8、16倍。在16倍剂量组,胚胎的平均头长为2.07mm、干重为0.53mg、发育评分为26.1,均显著低于空白对照组(P<0.05),并出现畸形胚胎。结论高浓度挥发性有机物对大鼠胚胎具有胚胎毒性和致畸性。Objective To study the developmental toxicity of indoor volatile organic compounds （VOC）. Methods 9.5- day rat embryos were transplanted and cultured for 48 hours in immediate centrifugal serum of rats, which had been exposed to VOC in an exposure cabinet for 10 days at the concentrations of 2 ,4 ,8,16 times respectively as high as the highest concentration tested in newly decorated rooms. At the end of a 48-hour cultural period, the embryos were removed from the culture and evaluated for their growth and development. Results Rat embryonic head length, embryo dry weight and developmental score in the concentrations of 16 times of exposure groups were significantly lower than those in the control groups （P 〈 0. 05 ）, and the dose also resulted in embryo abnormalities. Conclusion High concentrations of the volatile organic compounds may cause embryotoxicity and abnormalities in rats.

关 键 词：室内空气 挥发性有机物 全胚胎培养 胚胎发育 

分 类 号：R135.1[医药卫生—劳动卫生] R994.3[医药卫生—公共卫生与预防医学]