影响小麦组织培养效率的重要因素研究  

Important Factors Affecting Wheat Tissue Culture Efficiency

作　　者：赵永英[1] 李翠香[2] 苗红梅[1] 

机构地区：[1]河南省农作物新品种重点实验室,河南郑州450002 [2]河南工业大学粮油食品学院,河南郑州450052

出　　处：《河南农业科学》2007年第1期23-26,45,共5页Journal of Henan Agricultural Sciences

基　　金：河南省杰出人才创新基金项目(0121000700);河南省自然科学基金项目(0611032100)

摘　　要：以郑麦9023盾片为材料,对影响小麦组织培养的2,4-D,AgNO3,蔗糖进行了研究。结果表明,培养基中附加0.5 mg/L 2,4-D有利于胚性愈伤组织的诱导,添加5 mg/L和10 mg/L的AgNO3对根苗的分化有负效应,蔗糖30 g/L对根苗的分化最有利。In order to improve the efficiency of wheat tissue culture,the effects of 2,4 -D, AgNO3 and sucrose on callus induction and regeneration were studied by culturing the scutella from Zhengmai 9023. The results showed that the medium with 0.5 mg/L 2,4 -D was better for the inducement of embryogenic callus; additional 30g/L sucrose was beneficial to wheat green primor dium differentiation, while a supplement of 5mg/L or 10mg/L AgNO3 was negative.

关 键 词：小麦 组织培养 2 4-D AGNO3 蔗糖 

分 类 号：S512[农业科学—作物学]