高效液相色谱法测定海水中溶解态游离氨基酸  

High Performance Liquid Chromatographic Determination of Dissolved Free Amino Acids in Seawater

作　　者：杨桂朋[1] 李静[1] 高先池[1] 陆小兰[1] 

机构地区：[1]中国海洋大学海洋化学理论与工程技术教育部重点实验室,山东青岛266100

出　　处：《中国海洋大学学报（自然科学版）》2007年第1期125-128,134,共5页Periodical of Ocean University of China

基　　金：国家杰出青年科学基金(40525017);山东省"泰山学者"建设工程专项经费资助

摘　　要：采用邻苯二甲醛柱前衍生-高效液相色谱-荧光检测器（OPA—HPLC-FLD）联用技术分离测定了海水中的14种主要溶解游离氨基酸（DFAA），通过邻苯二甲醛-3-巯基丙酸（OPA-3-MPA）柱前衍生后，在XDB—C18柱上分离。不同氨基酸测定的精密度（RSD）为1．62％～3．80％，检测限为11．4～69．7ng·L^-1，海水加标回收率在92％～110％之间。利用该方法测得青岛近海表层海水中氨基酸浓度为0．015～0．284μmolL^-1。A system using reversed-phase high-performance liquid-chromatography coupled with fluorescence detection（RP-HPLC-FLD）has been developed for the determination of 14 main dissolved free amino acids in seawater. The free amino acids were derivatized with O-Phthalaldehyde and 3-mercaptopropionic acid （OPA-3- MPA）. The resulting OPA derivatives were then chromatographed on an XDB-C18 column. The measurement precisions for different amino acids were between 1.62 and 3.80% （relative standard deviation）; the detection limits were between 11.4 and 69.7 ng L^-1 ; the recoveries of standards added to seawater samples were between 92 and 110%. The amino acid concentrations in Qingdao coastal seawater determined with this system ranged（ from 0. 015-0. 284 μmol L^-10.

关 键 词：反相高效液相色谱法 邻苯二甲醛-3-巯基丙酸 溶解游离氨基酸 海水 

分 类 号：P342[天文地球—水文科学]