ACGM标记在竹子中的通用性  

Transferability of ACGM in Bamboo

作　　者：董德臻[1] 吴立成 夏善勇 王红蕾 卢泳全 

机构地区：[1]浙江省化工研究院,杭州310023 [2]黑龙江省农科学院

出　　处：《东北林业大学学报》2007年第1期4-6,共3页Journal of Northeast Forestry University

基　　金：国家"863"项目(2003AA207160)。

摘　　要：用39对ACGM引物对竹亚科5个属13个种进行PCR扩增,结果共有34对引物可以在至少一个竹种中获得特异性PCR产物。利用PHILIP软件,得到13个供试竹种材料的聚类图,总体上刚竹属是聚在一起的,而大明竹属的华丝竹和箭竹属的箭竹分别各自成类。其中有差别的是赤竹属的菲白竹和箬竹属的阔叶箬竹与刚竹属发生聚类在一起的情况。从刚竹属内部的聚类结果看,9个种可以大致分为4类,毛环竹、黄槽石绿竹、迟竹和刚竹为一类;毛芽竹和早园竹为一类;花竹和乌哺鸡为一类;红壳竹自成一类。他们之间的亲缘关系可以简单表示为:(((毛环竹+黄槽石绿竹+迟竹+刚竹)+(毛芽竹+早园竹))+(花竹+乌哺鸡))+红壳竹。本研究结果表明ACGM遗传标记可以有效地用于竹子的遗传分类及相关的分子生物学研究。The relationship between 13 accessions belonging to 5 genus of bamboo was investigated with 39 ACGM markers. A total of 34 pairs of primers exhibited specific PCR products in at least one species. Three pairs of primers （ GA1, GA7, GA28） could obtain PCR products in all the 13 bamboo species, but there were no polymorphisms between the products. According to different band styles of the rest 31 primer pairs, genetic distances between materials could be calculated. The 9 species of Phyllostachy were classified into four groups： Group l includes P. meyeri, P. arcana, P. bambusoides and P. sulphurea; Group 2 includes P. atrovaginata and P. propinqua; Group 3 includes P. nidulariaf and P. vivax; Group 4 includes P. rideseens only. The relationship between them could be showed simply as （ （ （P. meyeri, P. arcana, P. bambusoides, P. sulphurea） ＋ （P. atrovaginata, P. propinqua） ） ＋ （P. nidulariaf, P. vivax） ） ＋ P. ridescens. Results showed that ACGM can be well applied in genetic analysis and other related research in bamboo.

关 键 词：ACGM标记 竹子 通用性 

分 类 号：Q503[生物学—生物化学]