高效液相色谱法测定人血浆中奥沙普嗪浓度  

Determination of Concentration of Oxaprozin in Human Plasma Using HPLC

作　　者：陈开杰[1] 陈勇川[2] 戴青[2] 何菊英[2] 刘松青[2] 

机构地区：[1]重庆市肿瘤医院药剂科,重庆400030 [2]第三军医大学西南医院药学部,重庆400038

出　　处：《中国药业》2007年第3期50-51,共2页China Pharmaceuticals

摘　　要：目的建立奥沙普嗪血药浓度的高效液相色谱法(HPLC法)。方法血浆样品经甲醇直接沉淀蛋白进样,分析柱为Kromasil C18 100-5柱(250mm×4.6mm,5μm),流动相为甲醇-0.166mol/L磷酸二氢钠(80:20,pH=2.22),流速为1.0mL/min,检测波长为285nm。结果奥沙普嗪的线性范围为0.25～60μg/mL(r=0.9999),方法的日内和日间RSD<2.52%。结论该方法简单、快速、准确、灵敏、经济,适用于奥沙普嗪人体药代动力学研究。Objective To establish a HPLC method for determining the concentration of oxaprozin in human plasma. Methods The proteins were precipitated by adding methanol in the plasma samples. The supernatant was injected directly into the system. The analytical column was Kromasil C18 100 -5 （250 mm ×4.6 mm, 5 μm）, the mobile phase was methanol -0. 166 mol/L NaH2PO4 （80 ： 20,pH =2.22）, the flow rate was 1.0 mL/min, the detection wavelength was 285 nm. Results The linear range of oxaprozin was 0. 25 - 60 μg/mL（ r = 0.999 9）, the inter and intra day RSD were lower than 2.52%. Conclusion The method is simple, rapid, accurate, sensitive and economic. It can be used for studying pharmacokinetics of oxaprozin in human body.

关 键 词：奥沙普嗪 高效液相色谱法 血药浓度 

分 类 号：R969.1[医药卫生—药理学] R971.1[医药卫生—药学]