固相萃取分离苹果汁中富马酸的方法研究  

SOLID-PHASE EXTRACTION PROCEDURE TO ISOLATE FUMARIC ACID FROM APPLE JUICE

作　　者：唐长波[1] 岳田利[1] 

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100

出　　处：《食品研究与开发》2007年第2期120-123,共4页Food Research and Development

基　　金：国家"十五"科技攻关项目(2001BA501A15)

摘　　要：将固相萃取技术应用于苹果汁中富马酸的分离和富集。通过对固相萃取的主要影响因素进行研究,确定了最佳条件:用0.1mol/L氢氧化钠溶液调节果汁pH值至7.0,用4mL的0.1mol/L氢氧化钠活化柱子,取25mL的果汁上样,再用4mL去离子水淋洗柱子,最后用1.5mL0.1mol/L的硫酸溶液洗脱富马酸,将洗脱液直接上液相色谱检测。果汁样品中富马酸的回收率为60.8%～79.4%。A solid-phase extraction procedure was applied to isolate Fumaric acid from apple juice with an anion-exchange cartridge. The different parameters which affected the extraction procedure were studied and optimized to establish the optimal conditions for maximum recovery of fumaric acid and minimum extraction of interferences. The optimized procedure used a cartridge which was activated with 4 mL of 0.1 mol/L sodium hydroxide solution. A 25 mL volume of apple juice solution （pH=7.0） was passed at a flow-rate. The cartridge was washed with 4 mL of water and fumaric acid was eluted with 1.5 mL of 0.1 mol/L sulfuric acid. This solution was injected directly into the chromatograph. The recovery of juice sample was satisfactory and ranged from 60.8 % to 79.4 %.

关 键 词：固相萃取 富马酸 影响因素 回收率 

分 类 号：TS255.44[轻工技术与工程—农产品加工及贮藏工程]