产紫杉醇类云南红豆杉内生真菌筛选的研究  

Study on Endophytic Fungi Producing Taxol Isolated from Taxus Yunnanensis

作　　者：刘佳佳[1] 龚汉祥[1] 杨栋梁[1] 陈淑娟[1] 杨磊[1] 

机构地区：[1]中南大学化学化工学院,湖南长沙410083

出　　处：《现代生物医学进展》2006年第12期53-55,共3页Progress in Modern Biomedicine

摘　　要：采用无菌技术从云南红豆杉中分离得到200余株内生真菌,经过PDA液体培养基发酵后用TLC、HPLC等方法检测,发现其中有一株的胞外分泌物中含有紫杉醇,标号12．3．2。经过形态学鉴别,其属于青霉属(Penicillium．)。利用微生物(特别是内生真菌)产紫杉醇是新的途径,该菌株具有进一步研究的价值。More than 200 strains of endophytic fungi were isolated by aseptic techniques from the Taxus Yuimanensis in Yurman Province. They were cultured in the liquid potato medium and indentified by means of the thin layer chromatography （TLC） and HPLC. The result showed one strain of fungus which was signed 12.3.2 could produce taxol in vitro,according to its morphology, which belonged to penicillium.. Using microbe （especially the fungi） to produce Taxol is considered to be a new way to obtain Taxol.So this strain is worthy to be further studied.

关 键 词：云南缸豆杉 内生真菌 紫杉醇 

分 类 号：Q55[生物学—生物化学] S567[农业科学—中草药栽培]