重组表达载体pGEM-VLA4的构建  

Construction about Recombination Expression Vector of pGEM-VLA4

作　　者：安利峰[1] 胜利[1] 马力杨[1] 

机构地区：[1]西北民族大学医学院,甘肃兰州730030

出　　处：《西北民族大学学报（自然科学版）》2006年第4期41-43,共3页Journal of Northwest Minzu University(Natural Science)

基　　金：西北民族大学中青年科技基金项目(D2005-010)

摘　　要：从人外周血细胞中提取mRNA,用RT-PCR法扩增含BamH与HindⅢ酶切位点VLA4基因序列,运用分子生物学方法将该序列克隆到pGEM中,构建成VLA4基因序列的真核表达载体并进行鉴定分析.对重组载体用双酶切系统酶切后进行电泳并测序,结果证明目的基因克隆及连接方向正确,测序结果正确.通过实验,成功地构建了含VLA4基因的真核表达载体pGEM-VLA4.Objective： to construct recombination expression vector of pGEM- VLA4. Method： The mRNA of VLA4 was extracted from human peripheral blood cells- - V.LA4 rearrangement gene containing BamH and Hind 111 endoenzyme sites were obtained by using RT - PCR method. Molecular biological method was used to clone the gene to pGEM. Results： The recombination expression vector were cut by the system of double enzyme cuting. Target gene cloning and linking direction were proved to be correct, and the result of sequence measurement was correct. Conclusion： This study successfully constructed recombination expression vector of pGEM- VLA4.

关 键 词：载体 VLA4 构建 

分 类 号：Q784[生物学—分子生物学]