多环芳烃荧蒽降解菌的筛选鉴定及降解特性研究  

Studys on Isolation Identification of a Fluoranthene-degrading Strains and Its Degradation Characteristics

作　　者：杨晓磊[1] 陆贻通[1] 曹林奎[1] 

机构地区：[1]上海交通大学农业与生物学院,上海201101

出　　处：《科技通报》2007年第1期46-51,共6页Bulletin of Science and Technology

基　　金：国家973项目(2004CB41850);国家自然科学基金项目(20337010);上海市基础研究重点项目(04JC14051)

摘　　要：采用选择性富集培养方法,从东北老工业区的石油污染土壤中分离到能以高浓度荧蒽为唯一碳源和能源,并且生长良好的优势菌JU1。通过16s rDNA核苷酸序列分析以及生理生化鉴定此菌株为克雷伯氏菌属(Klebsiella sp.)。土样混合菌和优势菌株JU1都有较强的降解荧蒽的能力,使用高效液相分析方法测定荧蒽的含量,经过6 d混合菌株的荧蒽降解率为94.12%,优势菌株JU1能够达到降解率为92.71%。不同荧蒽浓度条件下,菌株JU1对荧蒽的降解率会受到较大的影响,在50 mg.L-1具有最好的降解效能。降解菌JU1还能对其他多环芳烃起到降解作用,对菲的降解率为40.76%,对苯并[a]芘降解率为62.38%。菌株JU1对荧蒽和菲混合多环芳烃的降解率第5 d后,分别为荧蒽59.74%和菲55.36%。The predominant indigenous microbial population and strain JU1 PAHs-degrading was obtained by selective enrichment culture from DongBei old industrial estate contaminated oil. The bacteria were Obtained with Ftuoranthene as the solo carbon source for growth. The bacteria were identified as Klebsiella sp. based on the sequence analysis of 16s rDNA and its morphological and physio-biochemcial characteristics. In a mineral salts medium with 50 mg·L^-1 fluoranthene, the microbial population and the strain could degradate fluoranthene well. 92.71% of fluoranthene in the medium were degraded in 6 days. Study on concentration was found that the degradation etticieney was the better than other concentrations in the 50 mg·L^-1. The degradation of Phenanthene could be 40.76% and Benzo [a]pyrene of also could be reached 62.35%. After 5 days, in the mixture solution the degradation rate of Fluoranthene was 59.74% and Phenanthene was 55.36%.

关 键 词：多环芳烃 降解 16S RDNA 克雷伯氏菌属 

分 类 号：Q935[生物学—微生物学]