HPLC-UV法同时检测犬血浆中异甘草酸镁及甘草次酸  

Simultaneous measurement of magnesium isoglycyrrhizinate and glycyrrhetic acid in plasma of dog by HPLC

作　　者：刘苏怡[1] 王庆敏[1] 曹聪[1] 丁建花[1] 胡刚[1] 

机构地区：[1]南京医科大学药理学系

出　　处：《中国临床药理学与治疗学》2006年第12期1395-1400,共6页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的：建立同时检测犬血浆中异甘草酸镁（MGL）及甘草次酸（GA）的高效液相紫外检测法（HPLC-UV）。方法：采用HPLC-UV法，检测波长252nm，Kromasil ODS-1 C18色谱柱，柱温25℃.流动相：乙腈-0．1％二乙胺水溶液（pH4．60），二元梯度洗脱，流速1．0ml·min^-1，Millennium^32工作站采集数据，外标法计算血药浓度。结果：血浆中MGL、GA峰分离良好，内源性物质不干扰样品峰。最低定量浓度为0．2mg·L^-1，两者浓度范围在0．2～100mg·L^-1呈线性相关；相对回收率分别为94．3％～101．9％和96．4％～101．9％，绝对回收率分别为78．7％～87．0％和77．5％～87．7％，日内和日间变异均小于15．0％；血浆样品-20℃冰箱中冻存14d、冻融3次、室温放置10h均稳定，提取后样品4℃放置9h稳定。结论：本实验方法具有专属性，灵敏可靠，适用于MGL制剂给药后血药浓度的检测。AIM： To quantify magnesium isoglycyrrhizinate（MGL） and glycyrrhetic acid in the plasma of dog by develop a simple, rapid, sensitive high-perfomlance liquid chromatography （ HPLC-UV ） method. METHODS： HPLC-UV methods with wavelength 252 nm were used for the quantitation of MGL and GA in plasma. The concentration of MGL and GA were assayed on a Kromasil ODS-1 C18 column with the column temperature 25 ℃. The mobile phase was a gradient system with 0.1% diethylamine in water （pH 4.60） and acetonitrile at a flow rate of 1.0 ml·min^-1. RESULTS： There was a good linear response range of 0.2 - 2.5 mg·L^-1 and 2.5 - 100 mg· L^-1, while the limit of quantification was 0.2mg· L^-1. The relative recovery rate of MGL was 94.3 % -101.9%,GA%.4%- 101.9% （n=5）;the absolute recovery rate was MGL 78.7% - 87.0%, GA 77.5 % - 87.7 % （ n= 5）. The intra-day and inter-day variations were all less than 15% （ n= 5）. The plasma samples preserved in refrigerator is stable at - 20 ℃ for 14 days, freeze thawing 3 times and at room temperature for 10 h without degradation. CONCLUSION： This method is shown to be specific, sensitive, reliable and suitable for the quantitative detemaination of magnesium isoglycynlaizinate following oral administration in biological sample.

关 键 词：异甘草酸镁 甘草次酸 高效液相紫外检测法 梯度洗脱 

分 类 号：R969.1[医药卫生—药理学]