大鼠实验性脑挫伤后不同时间脑apoE的表达  

The timing expression of apo E following experimental traumatic brain injury in the rat

作　　者：何光龙[1,2] 孙来晶[1] 周亦武[1,3] 贾冬梅[1] 邓伟年[1] 戴甲培[4] 刘良[1] 徐小虎[5] 

机构地区：[1]华中科技大学同济医学院法医学系 [2]公安部物证鉴定中心北京100038 [3]汕头大学医学院法医学教研室,广东汕头515031 [4]华中科技大学同济医学院神经生物学教研室 [5]汕头大学医学院法医学教研室

出　　处：《中国法医学杂志》2007年第1期11-14,共4页Chinese Journal of Forensic Medicine

基　　金：国家自然科学基金(30570629);中国博士后科学研究基金(2003034459);湖北省自然科学基金(2003ABA140)

摘　　要：目的观察大鼠实验性脑挫伤后脑内apo E蛋白变化的时空性规律。方法采用自由落体撞击法致大鼠脑挫伤模型，观察伤后不同时间（0．5h、2h、6h、12h、24h、3d、7d、14d）apo E蛋白的变化，并用计算机图像分析技术作定量统计分析。结果伤后0．5h组脑皮质神经元apo E出现阳性表达，3d达到高峰，随后下降；伤后0．5h组在海马出现apo E强阳性反应，CA1区在伤后3d达到高峰，而CA2～CA4区在24h达到高峰，随后逐渐下降，伤后海马apo E表达以CA1区最强。结论挫伤后apo E时空性变化规律可作为脑损伤时间推断、早期诊断及生前伤和死后伤鉴别的一个新的参考指标。Objective To Study the expression of apo E protein changed in different wound age and position of the experimental traumatic brain injury （TBI） in rat. Methods The animal model of cerebral contusion was established by impact to the parietal lobe with a free fall weight, observed the changes of apo E in different wound age （0.5h,2h,6h, 12h,24h,3d,7d, 14d）. The results were measured quantitatively with computer imaging analysis system. Results In cortex apo E-positive neurons definitely detected in 0.5h after brain injury, reaching the peak in 3d, then it shows the gradual decrease from 3d to 14d; In hippocampus apo E-positive neurons definitely detected in 0.5h after brain injury, reaching the peak in 3d in CA1 section and 24h in CA2 ～ CA4. Then it shows the gradual decrease. We found the expression of apo E protein in CA1 section is stronger than others. Conclusion The location and intersity of the immunoreactivity of apo E protein changed at the different stages after TBI. These changes observed in the present study might be used for determination of injury time, early diagnosis and distinguish antemortem and postmortem brain injury in forensic medicine.

关 键 词：法医病理学 脑挫伤 载脂蛋白E(apo E) 损伤时间 计算机图像分析技术 

分 类 号：D919[医药卫生—法医学]