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作 者:王容燕[1] 王金耀[1] 宋健[1] 曹伟平[1] 杜立新[1] 冯书亮[1] 宋福平[2] 张杰[2]
机构地区:[1]河北省农林科学院植物保护研究所,保定071000 [2]中国农业科学院植物保护研究所,北京100094
出 处:《昆虫学报》2007年第1期20-24,共5页Acta Entomologica Sinica
基 金:国家自然科学基金项目(30200184);河北省自然科学基金项目(C2005000642);河北省农林科学院青年基金项目
摘 要:对室内饲养铜绿丽金龟AnomalacorpulentaMotschulsky的方法和主要影响因子进行了研究。结果表明:室内饲养的铜绿丽金龟种群完成一个世代平均为272·3天,3龄幼虫期比田间种群缩短了103·8天。在饲养过程中,幼虫的生长容易受到土壤湿度、食料、病原微生物的影响。卵的孵化率为86·0%,1龄、2龄和3龄幼虫的存活率分别为82·0%、76·0%和60·0%。10和15日龄幼虫在含有马铃薯的土壤中饲养14天后的死亡率分别为8·8%±1·6%和4·0%±1·6%,明显低于初孵幼虫及5日龄幼虫的死亡率,可以作为生物测定的最佳供试幼虫。紫外线处理的壤土和沙壤土中铜绿丽金龟幼虫可以正常生长,死亡率较低。Rearing of Anomala corpulenta Motschulsky were conducted in the laboratory condition and the main influencing factors were evaluted. The results showed that it took 274.3 days for the beetle to complete a life cycle in the laboratory conditions. The duration of the 3rd instar reared in the laboratory was 101.8 days shorter than that in fields. The growth of larvae was influenced by such factors as soil humidity, food and pathogens. The average survival rate of eggs was 86.0% while those of the 1st instar, 2nd instar and 3rd instar larvae were 82.0%, 76.0% and 60.0%, respectively. The mortalities of the 10 day-old and 15 dayold larvae reared in the soil with potato as food for 14 days were 8.8% ± 1.6% and 4.0%± 1.6% respectively, significantly lower than those for the newly hatched larvae and 5 day-old larvae. This result suggested that the larval age of 10 - 15 day-old was the optimum stage for bioassay of Bt. The larvae grew normally in the UV-irradiated loam and sandy loam soil, with their mortalities lower than that in the untreated and sterilized loam and sandy loam soil.
分 类 号:S476.11[农业科学—农业昆虫与害虫防治] S433.2[农业科学—植物保护]
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