感染家蚕浓核病毒BmDNV1对家蚕抗性和敏感品种生化参数的影响(英文)  

Effect of BmDNV1 infection on the biochemical parameters in Densonucleosis type1 resistant and susceptible breeds of silkworm,Bombyx mori L.

作　　者：Monir SIRAJ M.BALAVENKATASUBBAIAH K.CHANDRASEKHARAN B.NATARAJU S.D.SHARMA T.SELVAKUMAR P.SUDHAKARARAO 

机构地区：[1]Central Sericultural Research and TrainingInstitute Srirampura, Mysore-570008 ,India [2]Central Sericultural Research and TrainingInstitute Srirampura, Mysore-570008,India

出　　处：《昆虫学报》2007年第1期74-78,共5页Acta Entomologica Sinica

摘　　要：在家蚕疾病中,病毒性疾病是危害茧产品的主要且最普遍的疾病。病毒性蚕软腐病是由BmIFV,BmDNV1和BmDNV2引起的。对印度种质库(Indian Germplasmstock)中的BmDNV1有抗性和敏感的家蚕品种进行了鉴定。利用标准方法对在BmDNV1感染过程中抗性和敏感品系的主要有机成分的变化(包括总蛋白、碳水化合物和脂类)进行了检测。结果表明:随着幼虫年龄的增长,对照组和处理组中有机成分(即蛋白质和碳水化合物)的含量也随之增长,但是处理组的增长水平明显地低于各自对照组的增长水平。接种BmDNV1后,与对照组比较,敏感品种体内的血淋巴和中肠组织的总蛋白的量显著地下降。在抗性品种,接种4天后的总蛋白的量有了显著地下降,之后,下降水平小于各自的对照组。在抗性和敏感品种中,血淋巴和中肠组织中的总碳水化合物的量略有下降。在变化不显著的抗性和敏感品种中,血淋巴和中肠组织中的脂质的量有显著地提高。在敏感家蚕品种,生化变化清晰地显示:BmDNV1感染消耗作为主要能量来源的总蛋白和碳水化合物。这些成分的损耗导致了被感染家蚕的生长受阻。Among silkworm diseases, viral diseases are the major and most common which damages the cocoon production. Viral flacherie is caused by BmIFV, BmDNV1 and BmDNV2. Resistant and susceptible silkworm breeds against BmDNV1 were identified among the Indian Germ plasm stock. The changes in major organic constituents viz., total protein, carbohydrate and lipid content during the progressive infection of BmDNV1 in susceptible and resistant breeds were estimated as per the standard methods. As the larval age increases, there was a consistent increase in the level of organic constituents viz., protein and carbohydrates in both control and treated batches, but the level of increase in treated batches were markedly less when compared to the respective control batches. After inoculation with BmDNV1, there was a significant decrease in total protein content in haemolymph and mid gut tissue in susceptible breeds when compared with control batches. In resistant breeds, there was a significant decrease in total protein content up to 4 day post inoculation （PI） hut subsequently, the decrease was less compared to the respective controls. There was a slight decrease in total carbohydrate content in haemolymph and mid gut tissue in susceptible and resistant breeds. In case of lipid content, there was a significant increase in haemolymph and mid gut tissues in susceptible breeds and in resistant breeds the changes were not found significant. In susceptible silkworm breeds, the biochemical changes clearly indicated that the BmDNV1 infection depletes the major organic constituents viz., total protein and total carbohydrate contents which are the major sources energy. This depletion in these contents resulted in stunted growth in infected silkworms.

关 键 词：家蚕 家蚕浓核病毒 总蛋白 总碳水化合物 总脂类 抗性品系 敏感品系 

分 类 号：Q966[生物学—昆虫学]