不同寄主蒲螨种群间亲缘关系的RAPD初步分析  

The RAPD analysis on the phylogeny of Pyemotes spp. on different hosts

作　　者：韩继成[1] 于丽辰[1] 贺丽敏[1] 郝宝峰[1] 许长新[1] 

机构地区：[1]河北省农林科学院昌黎果树研究所,河北昌黎066600

出　　处：《昆虫学报》2007年第1期90-94,共5页Acta Entomologica Sinica

基　　金：国家自然科学基金(397706222);河北省自然科学基金(302446)

摘　　要：应用RAPD技术分析了采自河北昌黎和天津蓟县等不同地点的9个不同寄主的蒲螨种群间的关系。通过对100条RAPD引物的3次重复筛选，获得32条重复性好、多态性高的引物。应用这32条引物共扩增出268条带，依据这些条带计算遗传距离，再进行聚类分析。结果表明：9个蒲螨种群可分为3个组（A、B和C组）。其中A和B组属于小蠢蒲螨群，A组由护林神蒲螨Pyemotes dryas组成；B组由小蠢蒲螨P．scolyti和寄生于松树小蠢Scolytidae sp.的蒲螨组成；球腹蒲螨群组成C组，并分为两个分支，寄生于桃树小蠢寄生蜂Pteromalidaespp．的蒲螨为一支，寄生于柏肤小蠢Phloeosinusaubd Perris、皮蠢Derrnestidae sp．、日本二齿茎长蠢Sinoxylon japonicum Lesne（黑枣树和柿树）和桑梢小蠢Cryphalus exignus 等的5个蒲螨种群为一支，这5个蒲螨种群形态上未见明显差别。C组中的寄生桃树小蠢寄生蜂的蒲螨与该组其他蒲螨种群的遗传距离最远，达到0．3236-0．4111，并且存在生殖隔离，该蒲螨似已分化成为独立的新种；C组其他种群是否存在近缘种，有待进一步深入研究。The Pyemotes is one of the most important natural enemies of the bark pests, and spreads widely in China. It is difficult to identify the relationships among the Pyemotes populations on different hosts because of their belonging to closely related species undistinguishable by morphological characters. The RAPD analysis of 9 populations of Pyemotes on different hosts collected from Changli County, Hebei Province and Jixian County, Tianjin City were carried out to explore the relationships among these Pyemotes populations. Thirty-two primers with high polymorphic and steady bands were obtained after selecting in 100 primers repeatedly. The results of phylogeny analysis based on the genetic distances of the tested 9 populations indicated that the they could be divided into three groups （A, B and C）. Group A contains Pyemotes dryas only; Group B contains P. scolyti and the Pyemotes parasitizing Scolytidae spp. ; Group C are divided further into two subgroups, one is the Pyemotes parasitizing Pteromalidae spp., and another contains the Pyemotes parasitizing Phloeosinus aubei Pert＇is, Dermestidae sp., Sinoxylon japonicum Lesne, and Cryphalus exignus, respectively. Reproductive isolation was found between the population of Pyemotes parasitizing Pteromalidae spp. and others, which suggests that the former should be a different new species.

关 键 词：蒲螨 寄主 分化 RAPD 亲缘关系 

分 类 号：Q969[生物学—昆虫学]