参附注射液对大鼠心肌缺血再灌注损伤的保护作用  

Protective Effect of Shen-fu on Myocardial Ischemia-reperfusion Injury in Rats

作　　者：吴永涛[1] 罗毅[1] 顾云[2] 苏俊武[1] 辛毅[2] 

机构地区：[1]首都医科大学附属北京安贞医院小儿心脏外科,北京100029 [2]北京市心肺血管疾病研究所分子生物学研究室,北京100029

出　　处：《中国比较医学杂志》2007年第1期26-29,I0002,共5页Chinese Journal of Comparative Medicine

基　　金：卫生部999中药注射液科研基金资助项目(200307)

摘　　要：目的探讨中成药参附注射液对在体结扎大鼠冠状动脉缺血-再灌注心肌损伤的效果以及作用机理。方法将46只SD大鼠随机分成5组,(1)非手术组;(2)结扎左冠状动脉前降支(LAD)引起心肌缺血30 min对照组1;(3)心肌缺血30 min给药组1;(4)心肌缺血/再灌注10 min对照组2;和(5)心肌缺血/再灌注10 min给药组2。分别测定心肌组织匀浆丙二醛(MDA)含量,应用透射电镜观察心肌细胞超微结构改变,并对线粒体进行体视学分析,以及抗氧化基因超氧化物岐化酶1(SOD1)和谷胱苷肽S转移酶基因的表达。结果给药组与对照组同一时间点比较心肌组织MDA含量均有显著下降(P<0.05);超微结构显示参附明显减小心肌细胞组织结构以及线粒体的损害;体视学分析显示对照组较非手术组线粒体有显著变化(P<0.01),而两给药组较非手术组线粒体变化较小(P<0.05);参附上调SOD1和谷胱苷肽S转移酶基因的表达。结论参附注射液通过保护线粒体,减轻脂质过氧化的程度;还可通过上调SOD1及谷胱苷肽S转移酶等抗氧化基因,增加机体抗氧化能力抵抗缺血/再灌注时过氧化脂质损伤,保护心肌细胞。Objective To study the protective effect and the mechanism of anti-myocardial-ischemia-reperfusion injury of Shen-fu injection through ligating the left anterior descending（LAD） of rats in vivo. Methods The 46 rats were divided into 5 groups randomLy： （1） no-operation group; （2） the control group 1 ： 30-minute ischemia; （3） pretreatment group 1 with Shen-fu： 30-minute ischemia; （4） the control group 2： 30-minute ischemia followed by 10-minute reperfusion; （5） pretreatment group 2 with Shen-fu： 30-minute ischemia followed by 10-minute repeffusion. The content of malondialdehyde（MDA） and the expression of antioxidant genes of superoxide dismutase 1 （SOD1） gene and glutathione S-transferase gene were measured, the ultrastructure of the myocardial cells by transmission electron microscope was observed. The mitochondrial stereology analysis was done. Results The MDA of the pretreatment groups with Shen-fu was powful decreased compare with the control groups. The significant decreasing the lesion of the myocardial cell and mitochondfial in ultrastructure were found in the pretreatment groups with Shen-fu. The stereology analysis display that the mitochondrial in the control groups have significant changed compare with the no-operation group. And the mitochondrial in the pretreatment group with Shen-fu was a little different from the no-operation group. Shen-fu upregulated the expression of SOD1 gene and glutathione S-transferase gene. Conclusion The Shen-fu protect the myocardial cell by reducing the lipid peroxidation. Their protective effects may be partly related to up-regulating the antioxidant gene that increase the ability of antioxidant.

关 键 词：参附注射液 缺血-再灌注损伤 心肌保护 

分 类 号：R654.1[医药卫生—外科学]