弓形虫复合黏膜疫苗滴鼻免疫小鼠诱导NALT和GALT黏膜部位的免疫应答  

Intranasal Immunization with Mucosal Complex Vaccine for Toxoplasma gondii Induces Local Immune Responses in Both NALT and GALT

作　　者：李珀[1] 孟晓丽[1] 殷国荣[1] 张杰[1] 

机构地区：[1]山西医科大学医学寄生虫学研究所

出　　处：《热带医学杂志》2007年第1期3-6,共4页Journal of Tropical Medicine

基　　金：国家自然科学基金(No.30640057);山西省自然科学基金(No.20041105);山西省高校科技研究开发项目(No.20041238);山西医科大学基础医学院"学科研究方向建设基金"(No.20040708)。

摘　　要：目的观察弓形虫复合黏膜疫苗滴鼻免疫小鼠诱导的鼻相关淋巴组织(NALT)和肠相关淋巴组织(GALT)黏膜部位的免疫应答,探讨弓形虫黏膜疫苗能否在感染起始阶段有效诱导免疫应答。方法将BALB/c小鼠随机分为免疫和对照组,免疫组小鼠以弓形虫复合黏膜疫苗20μl/只(20μgSTAg+1μgCT)滴鼻免疫2次(间隔2周),对照组PBS滴鼻。于末次滴鼻后14d颈椎脱臼处死小鼠,ELISA测定粪便和鼻咽冲洗液IgA抗体水平;计数NALT、NC及PP、IEL淋巴细胞数,免疫细胞化学法检测CD4+、CD8+T细胞亚群水平。结果免疫组小鼠粪便IgA和鼻咽冲洗液IgA抗体显著高于对照组(P<0.05)。NALT内淋巴细胞明显增生(P<0.05),其中CD4+、CD8+T细胞均有增生(P<0.01),CD4+/CD8+比值降低(P<0.05)。NC中淋巴细胞数明显升高(P<0.01),以CD4+T细胞水平为主(P<0.01)。GALT部位PP淋巴细胞增生显著(P<0.01),主要以CD4+T细胞为主;而IEL以CD8+T细胞增生为主(P<0.01),CD4+/CD8+比值降低(P<0.05)。结论弓形虫复合黏膜疫苗滴鼻免疫BALB/c小鼠可有效诱导NALT和GALT黏膜部位免疫应答。Objective To study the local immune response in NALT and GALT after intranasal immunization with mucosal complex vaccine for Toxoplasma Bond/i, and to determine whether intranasal （i.n） immunization is an effective route for Toxoplasma gondii mucosal vaccine. Methods BALB/c mice were randomly divided into two groups： immunized group and control group. Mice were immunized （i.n） with 20 μl mucosal complex vaccine （20 μg STAg＋1μg CT） twice at an internal of two weeks, while the control mice were given with PBS. Mice were sacrificed on day-14 after the last immunization. IgA antibodies in feces and nasopharynx washes were detected by ELISA. Lymphocytes in NALT,NC,PP and IEL were isolated and counted. Percentage of CD4^＋ and CD8^＋ T cells was determined by imm,mocytochemistry. Results IgA antibodies in feces and nasopharynx washes of immunized mice were higher than the control group （P〈 0.05）. The number of lymphocytes in NALT was significantly increased after immunization （P〈0.05）. The numbers of CD4^＋ and CD8^＋ T cells were both higher than the control （P〈0.01）, while the ratio of CD4^＋ to CD8^＋ T was decreased （P〈0.05）. The number of lymphocytes in NC and PP significantly increased （P〈0.01）, and the increase was mainly due to the increase of CD4^＋ T ceils （P〈0.01）. For IEL, there was a predominant number of CD8^＋ T increasing significantly （P〈0.01）, and the ratio of CD4^＋ and CD8^＋ T cells was reversed with significance （P〈0.05）. Conclusion Intranasal immunization with mucosal complex vaccine for Toxoplasma gondii can effectively induce immune responses into both NALT and GALT compartments. And intranasal immunization is an effective route for Toxoplasma Bondii mucesal vaccine.

关 键 词：弓形虫 黏膜疫苗 滴鼻免疫 鼻相关淋巴组织 肠相关淋巴组织 

分 类 号：R382.5[医药卫生—医学寄生虫学]