水稻胆碱单加氧酶基因的克隆与表达分析(英文)  

Molecular Cloning and Expression of OsCMO Encoding a Putative Choline Monooxygenase in Rice (Oryza sativa L.)

作　　者：潘丽娟[1] 黄骥[1] 王州飞[1] 张红生[1] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室,南京210095

出　　处：《分子植物育种》2007年第1期8-14,共7页Molecular Plant Breeding

基　　金：This work was supported by Natural Science Foundation of China (Grant No.30571141);Natural Science Foundation of Jiangsu province (Grant No.BK2005090);Ph.D program foundation from Ministry of Education (20060307035);Changjiang scholars and innovative research team in university (PCISRT).

摘　　要：高等植物中甜菜碱由胆碱经两步氧化反应合成，其中由胆碱单加氧酶（CMO）催化第一步氧化反应，甜菜碱醛脱氢酶（BADH）催化第二步氧化反应。采用生物信息学技术和RT-PCR的方法从水稻幼苗组织中分离得到了水稻CMO基因的eDNA克隆，将其命名为OsCMO。该基因含有10个外显子和9个内含子，其开放阅读框编码一条长为410个氨基酸残基的多肽，与拟南芥、山菠菜、苋、碱蓬和甜菜等植物的胆碱单加氧酶的氨基酸序列的一致性50％-62％。mRNA分析表明OsCMO基因在水稻幼苗组织中的表达受高盐、低温和干旱等胁迫的上调诱导表达，表明该基因可能在抵御非生物胁迫中发挥重要作用。In plants, the osmoprotectant glycine betaine （GB） is synthesized via the pathway that choline monooxygenase （CMO） oxidizes choline to betaine aldehyde, which is subsequently oxidized to GB by betaine aldehyde dehydrogenase （BADH）. Although rice is considered as a typical non-glycinebetaine accumulating species, a eDNA encoding putative choline monooxygenase （CMO） was isolated from rice by RT-PCR and designated as OsCMO. The OsCMO contains 10 exons and 9 introns and encodes a protein with 410 amino acids, which shows 62%, 51%, 51%, 51% and 50% identities in amino acids to CMOs from Arabidopsis thaliana, Atriplex hortensis, Amaranthus tricolor, Suaeda liaotungensis and Beta vulgaris respectively. Semi-quantitative RT-PCR assay revealed OsCMO was significantly up-regulated in the rice seedlings under salt, cold and drought stresses. It suggests that OsCMO might play an important role in rice responses to abiotic stresses.

关 键 词：水稻 胆碱单加氧酶基因 基因克隆 RT-PCR 表达特性 非生物胁迫 

分 类 号：S511[农业科学—作物学] Q943.2[生物学—植物学]