一个水稻半矮秆突变体的鉴定与遗传分析  

Identifi cation and Genetic Analysis of a Rice Semi-dwarf Mutant

作　　者：龙美西[1] 王玉平[1] 石军[1] 陈家彬[1] 李仕贵[1] 

机构地区：[1]四川农业大学水稻研究所

出　　处：《分子植物育种》2007年第1期59-63,共5页Molecular Plant Breeding

基　　金：教育部新世纪优秀人才支持计划(NCET-04-0907);教育部创新团队发展计划(IRT0453)资助。

摘　　要：高黄矮材料是由黄矮与高秆材料杂交后代F2群体中分离得到的一个半矮秆突变体材料,株高在95cm左右,比黄矮高约40cm。该材料在苗期倒2叶叶尖开始出现黄化,分蘖期倒2叶叶尖仍然黄化,倒3叶近1/3叶面黄化干枯、破损现象比较明显。将该突变材料与不同高秆材料杂交,杂种F1表现为高亲本,F2群体株高数据符合3:1分离比例,结果表明该矮生性状受1对隐性基因控制,因其叶尖黄化性状与半矮秆性状共分离,故暂将该基因命名为dy(t)基因;同时将突变材料与其他不同基因型的半矮秆杂交,F2群体均符合9:3:3:1的分离比例,结果表明dy(t)基因与sd1、sdn、sdg基因均不等位,为一新的半矮秆基因。对不同株高材料的幼苗进行不同浓度外源赤霉素(GA3)叶面喷施处理后,结果表明dy(t)基因对该激素敏感。A tentative new rice semi-dwarf germplsam, Gao Huang Ai, identified in the F2 progenies derived from Huang Ai mating with an anonymous high culm germplasm. The mutant has 95 cm culm higher than that of Huang Ai about 40 cm. The unique phenomenon of the yellowish leaf starts at seedling stage and continues at tilling stage. In order to make clear the genetic base of the mutant, we made the combinations between the mutant and several high stalk germplasms the phenotypes of F1 individuals were the same as the one of parent with tall stalk and the segregation ratio of the stalk phenotypes （tall to dwarf） in F2 population would be 3 to 1, which suggests that the dwarfing phenotype of Gao Huang Ai is controlled by one recessive semi-dwarf locus. Based on the yellowish phenotype of leaf finial co-segregated with the stalk height of the mutant, we tentatively defined the now semi-dwarf locus as dy（t）. In this research we conducted the allelism test using several known semi-dwarf germplasms, the allelism test indicated that the locus of the Gao Huang Ai should not be allelic to the locus sdl, san and sdg. The results also showed that the mutant of Gao Huang Ai was sensitive to gibberellin acid （GA3） based on the exogenous GA3 treatment.

关 键 词：水稻 半矮秆突变体 遗传分析 

分 类 号：S511[农业科学—作物学]