TNF-α基因多态性对雷公藤甲素抑制PBMC分泌TNF-α的影响  

The study of the relation between triptolide inhibits peripheral blood mononuclear cell to secret TNF-α and tumour necrosis factor-α gene polymorphism

作　　者：陈红波[1] 涂盛豪[2] 盛冬云[2] 胡永红[2] 

机构地区：[1]浙江省中医院免疫肾病科,杭州310006 [2]华中科技大学同济医学院附属同济医学院中医科,武汉430030

出　　处：《中国免疫学杂志》2007年第1期49-52,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金资助项目(90209049)

摘　　要：目的TNF-α基因多态性对雷公藤甲素刺激PBMC分泌TNF-α的影响。方法采用等位基因特异引物PCR法对41名健康志愿者TNF-α基因启动子区-308位点基因多态性进行检测,同时进行外周血单个核细胞(PBMC)培养,用脂多糖(LPS)或雷公藤甲素刺激培养细胞,收集上清液,ELISA法检测上清液中TNF-α的含量。结果TNF-α-308非G/G纯合子基因型健康志愿者PBMC经LPS刺激后TNF-α的分泌量明显较TNF-α-308G/G纯合子高;雷公藤甲素能够抑制TNF-α-308G/G纯合子基因型健康志愿者PBMC分泌TNF-α,而对TNF-α-308非G/G纯合子基因型健康志愿者PBMC没有明显的抑制作用。结论肿瘤坏死因子基因多态性与雷公藤甲素刺激外周血单个核细胞分泌TNF-α的量存在一定的联系,推测该基因多态性可能与临床雷公藤治疗类风湿关节炎所产生的个体疗效差异有一定关联。Objective：To study the relation between triptolide inhibit peripheral blood mononuclear cell to secret TNF-α and tumour necrosis factor-α gene polymorphism. Methods： Genomic DNA from 41 healthy people was typed for TNF-α -308 polymorphism by allele-specific polymorphism chain reaction（AS-PCR） ; the TNF-α concentration in the supernatant was measured by ELISA. Results：The TNF-α production of TNF-α -308 non-G/G genotype in LPS-inhibited peripheral blood mononuclear cell（PBMC） culture was more than that of G/G genotype; Compared with TNF-α -308 non-G/G genotype peripheral blood mononuclear cell（PBMC）, triptolide can lower the production of TNF-α in G/G genotype peripheral blood mononuclear cell（PBMC）. Condusion：Tumour necrosis factor a （TNF-α） gene polymorphism might influence the TNF-α secretion of peripheral blood mononuclear cell（PBMC） in healthy humans. We speculate that it may be relative to the different curative effect of Tripterygium Wilfordii Hook. F. （TWHF） to RA patients.

关 键 词：雷公藤甲素 肿瘤坏死因子 外周血单个核细胞 基因多态性 

分 类 号：R392[医药卫生—免疫学]