脐血CD34^+造血干/祖细胞基因表达图谱的研究  

Gene expression analysis of primary human CD34^+ hematopoietic stem and progenitor cells from umbilical cord blood

作　　者：李群良[1] 蔡海波[1] 刘启威[1] 谭文松[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《中国免疫学杂志》2007年第1期59-62,共4页Chinese Journal of Immunology

基　　金：上海市科技攻关项目资助(项目编号:05DZ19328)

摘　　要：目的通过脐血CD34+造血干/祖细胞的基因表达分析,理解造血干/祖细胞生物学特性。方法利用Min-iMACS免疫磁珠法从脐血细胞中分离CD34+造血干/祖细胞,提取总RNA,用SMART-PCR技术从微量RNA中扩增产生足够量的cDNA用于高密度点阵膜分析检测CD34+造血干/祖细胞表达的基因。结果在所检测的588个基因中,发现63个基因具有显著的表达水平,其中18个基因强表达。这些基因主要涉及造血干细胞增殖、分化、应激响应、凋亡、转录调节以及细胞周期等。结论对理解脐血干/祖细胞生物学性质以及指导造血干细胞体外培养提供了分子生物学基础。Objective：To better understand biological activities of CD34^＋ hematopoietic stem and progenitor cells（HSPCs） derived from umbilical cord blood（UCB）. Methods： CD34^＋ HSPCs were collected from fresh UCB by MiniMACS seperation. SMARTPCR technology was employed to amplify cDNA from isolated RNA. Hybridization experiments were performed to determine gene expression pattern of CD34 ^＋ HSPCs using Atlas eDNA expression array covering 588 genes. Results ：The data from gene expression analysis revealed that 63 genes were significantly expressed in CD34 ^＋ HSPCs, and 18 genes were expressed at high levels. These expressed genes involved mainly in proliferation, differentiation, stress responses, apoptosis, transcription regulation, and cell cycle. Condusion：This study gives some new insights into genetic program of HSPCs, and it may be helpful to understand biological properties and improve manipulation of hematopoietic cells.

关 键 词：脐血 CD34^+造血干/祖细胞 SMART—PCR 高密度点阵膜 

分 类 号：Q2[生物学—细胞生物学]