雏鸡感染鸡传染性贫血病病毒分布情况研究  

Study on the Distribution of CAV in 1-day Chickens

作　　者：刘泽文[1] 徐涤平[1] 杨峻[1] 汪宏才[1] 周平华[1] 段正赢[1] 邵华斌[1] 

机构地区：[1]湖北省农业科学院畜牧兽医研究所,武汉430209

出　　处：《湖北农业科学》2007年第1期113-114,共2页Hubei Agricultural Sciences

基　　金：湖北省科技攻关项目(2001AA201B03)

摘　　要：将鸡传染性贫血病毒强毒株C364和弱毒株C545分别接种1日龄鸡传染性贫血病毒抗体阴性的健康雏鸡,于接种后第7天和14天每组抽查3只测定血球压积值,并在接种后第1、3、5、7、10和14天各组分别宰杀3只雏鸡,取胸腺、肝脏和骨髓组织,用竞争性PCR测定其病毒含量。结果表明,雏鸡感染CAV,后表现为血球压积值下降,其中强毒感染鸡较弱毒感染鸡的红细胞压积值下降幅度大;病毒能够在雏鸡胸腺、肝脏和骨髓内繁殖,其中胸腺内病毒增殖最快,病毒滴度最高,肝脏其次,骨髓最低。High virulent CAV C364 strain and low virulent CAV C545 strain were inoculated respectively in 1-day chickens with negative CAV antibody. Hematocrit（HCT）of three chickens was determined at the 7^th and 14^th day. The CAV contents of the chicken thymus, liver and bone marrow were determined at the 1^st,3^nd,5^th,7^th,10^th and 14^th day using competitive PCR. The results indicated that HCT of 1-day chickens infected by strong virulent CAV decreased faster than those infected by low virulent CAV. CAV could propagate in chickens＇ thymus, liver, bone marrow. The titer of CAV in thymus is higher than liver and bone marrow.

关 键 词：鸡传染性贫血病毒 血球压积值 病毒动态分布 

分 类 号：S852.659.2[农业科学—基础兽医学] S854.4[农业科学—兽医学]