铅对大鼠海马CA1和CA3区Bcl-2阳性细胞影响  

Effects of lead on Bcl-2 positive cell in CA1 and CA3 of hippocampusin rats

作　　者：贺智[1] 李积胜[1] 王大宁[1] 刁秋霞[1] 何瑞波[1] 

机构地区：[1]武警医学院军事预防医学研究所,天津300162

出　　处：《中国公共卫生》2007年第2期197-198,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金(39870689)

摘　　要：目的了解海马各区Bcl-2阳性细胞在铅神经毒机制中的作用。方法采用免疫组化(ABC)法观察腹腔注射130 mg/kg醋酸铅溶液大鼠12和24 h后海马CA1区和CA3区Bcl-2阳性细胞数目的变化。结果染铅12和24 h大鼠海马CA1区Bcl-2阳性细胞数目(分别为126.05±33.21和(57.45±37.66)与对照组比较(201.94±31.72)明显减少(P>0.05,或P<0.01),但各组大鼠海马CA3区及其他组的CA1区Bcl-2阳性细胞数与对照组比较,差异无统计学意义(P>0.05)。结论染铅大鼠海马CA1区Bcl-2阳性细胞数目减少可能与铅的神经毒作用有关。Objective To explore the role of Bel-2 positive cells of different sections of hippocampus on lead - induced neurotoxlcity. Methods Immune histoehemistry was applied to observe variety of numbers of Bel-2 positive neurons in CA1 and CA3 of rats, which were caused by injecting renter irnus lead acetate of 130mg/kg. Results After 12h, the number of Bel-2 positive neurons of CA1 Of lead - induced rats was （ 126.05 ± 33.21） ;As for 24h, the number was （57.45± 37.66） ; Both of them were significantly less than that of control rats （201.94 ± 31.72） （ P 〈 0.05）. There were no difference between lead - induced rats and control one in CA3, neither were in CA1 （P 〉 0.05）. Conclusion The lead - induced neurotoxicity may attribute to decreasing numbers of Bel-2 positive neurons in CA1 of hippoeampus in rats.

关 键 词：铅 BCL-2表达 海马 

分 类 号：R995[医药卫生—毒理学]