八氯二丙醚对体外培养CHL细胞DNA合成影响  

Effects of octachlorodipropyl ether on DNA synthesis in CHL cells in vitro

作　　者：曾垂焕[1] 唐萌[1] 熊丽林[1] 王晓娜[1] 夏婷[1] 

机构地区：[1]东南大学公共卫生学院,南京210009

出　　处：《中国公共卫生》2007年第2期202-204,共3页Chinese Journal of Public Health

基　　金：江苏省预防医学基金(Y200421);铁道部科技基金(674780030);东南大学科技基金(XJ019005)

摘　　要：目的研究八氯二丙醚对体外培养中国仓鼠肺细胞(CHL)DNA合成影响。方法采用DNA荧光定量测定方法,观察不同染毒浓度(0.250,0.187,0.125,0.062,0.046,0.031μg/ml)对CHL细胞DNA合成影响。结果八氯二丙醚浓度为0.062,0.046μg/ml时DNA合成量与对照组比差异有统计学意义,表现为合成增加(P<0.05),浓度为0.250,0.187,0.125μg/ml时,则表现为合成抑制(P<0.05),且呈剂量-反应关系(P<0.05);0.250μg/ml浓度在脱离染毒后2,4,6,12 h内DNA合成量持续下降。结论在低浓度时(0.062,0.046μg/ml),八氯二丙醚可引起CHL细胞DNA合成能力增加,而在高浓度时(0.250,0.187,0.125μg/ml)则表现为DNA合成抑制,且这种抑制由DNA损伤引起。Objective To study effects of octachlorodipropyl ether on DNA synthesis in CHL in vitro. Methods Fluorimettle quantification assay was applied to detect DNA synthesis of CHL cells treated with different concentrations（ 0. 250, 0.187,0.125,0. 062, 0. 046, 0. 031μg/ml）. Results In the concentration gruop of 0. 062 and 0. 046μg/ml, DNA synthesis quantities were significantly different from the control group（ P 〈 0.05）, However, DNA synthesis inhibition and good dose - response relationships were shown in the gruop of 0. 250, 0.187 and 0. 125μg/ml. DNA synthesis quantities of 0. 250μg/ ml group indicated continual descent trend when it was treated without octachlorodipropyl ether in 2 -- 12 horus. Conclusion Low concentration of octachlorodipropyl ether could lead to the increase of DNA synthesis ability in CHL cells. Concentrations of 0.250, 0.187, and 0.125 μg/ml may lead to DNA synthesis inhibition, which was caused by DNA damage.

关 键 词：八氯二丙醚 中国仓鼠肺细胞 DNA合成 DNA荧光定世 

分 类 号：R114[医药卫生—卫生毒理学]