日本血吸虫Sjc97 DNA疫苗免疫小鼠攻击感染后的组织细胞反应  

Tissue reactions to schistosomula in C57BL/6 mice immunized with Sjc97 DNA vaccine after challenged with cercariae of Schistosoma japonicum

作　　者：陈家旭[1] 刘述先[1] 曹建平[1] 宋光承[1] 徐馀信[1] 

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所

出　　处：《中国血吸虫病防治杂志》2007年第1期17-20,共4页Chinese Journal of Schistosomiasis Control

基　　金：国家高技术研究发展计划(863计划)(2001AA215151)~~

摘　　要：目的观察日本血吸虫副肌球蛋白核酸疫苗（Sjc97DNA）免疫宿主攻击感染后的皮肤及肺组织反应。方法105只C57BL／6小鼠，随机分成Sjc97DNA疫苗组、空质粒对照组和感染对照组。前两组分别以Sjc97DNA核酸疫苗、空质粒经后腿胫前肌免疫C57BL／6小鼠共2次，每次间隔3周。末次免疫后3周攻击感染，3组小鼠经腹部皮肤攻击感染日本血吸虫尾蚴（800＋50）条。于攻击感染后6、18、24、48、72、96、120h，每组分别剖系5只小鼠，取腹部皮肤及／或肺组织作病理观察。结果Sjc97DNA疫苗免疫小鼠攻击感染血吸虫尾蚴后，皮肤炎症反应出现早，炎症细胞杀童虫现象明显，炎症反应强烈，且持续时间长，嗜酸性粒细胞浸润百分比高；而空质粒对照组和感染对照组皮肤炎症轻，持续时间短，杀虫现象不明显。Sjc97DNA疫苗免疫鼠攻击感染后肺部出血斑点出现时间（72h）迟于空质粒对照组和感染对照组（48h）。72～120h，Sjc97DNA疫苗组小鼠肺部局灶性炎症明显，肉芽肿样结节形成，但肺泡壁多正常；而空质粒组和感染对照组小鼠肺组织炎症轻，但肺泡壁水肿有较多红细胞渗入。结论Sjc97DNA疫苗免疫增强了小鼠皮肤及肺组织的细胞反应及其杀童虫作用。Objective To observe tissue reactions of skin and lung of mice immunized with Sjc97 DNA vaccine after challenged with cercariae of Schistosoma japonicum. Methods One hundred and five mice of C57BL/6 were divided into three groups, Sjc97 DNA group, blank plasmid group and infection control group, and 35 mice each group. The mice of the Sjc97 DNA group were vaccinated intramuscularly with Sjc97 DNA nucleic acid vaccine twice at an interval of 3 weeks, and then challenged with （800±250） cercariae of Schistosoma japonicum per mouse three weeks after final DNA boosting. The mice in the control groups, i. e. the blank plasmid and the infection control groups, were challenged with the same number of cercariae after vaccinating with plasmid or with nothing. Local skin and lung samples from the mice of each group were taken at certain time points from 6 to 120 hours after the infection for pathological observation. Results The skin inflammation in Sjc97 DNA immunized mice （Sjc97 DNA group） post challenge infection appeared early, and the cellular reactions against schistosomula in the skin tissues were acute and persistent, in which the percent age of eosinophils was high. However, in two control groups, the blank plasmid group and infection control group, the skin inflammation emerged lately, and the cellular reactions against sehistosomula were weaker than those in Sjc97 DNA group, The bleeding speckles on the lung’s surface of C57BL/6 mice immunized with Sjc97 DNA vaccine post-challenge infection appeared lately at 72 h after the challenge. In contrast, the bleed spots appeared early by 48 h after the infection in the blank plasmid group and infection control group. From 72 h to 120 h after the challenge infec tion, pulmonary inflammation was obvious, granulomas formed, but the most walls of pulmonary alveolus were normal in mice immunized with Sjc97 DNA vaccine. However, in two control groups, the pulmonary inflammation was weak but there were a lot of red blood cells seeping into the pulmonary alveolus wal

关 键 词：日本血吸虫 Sjc97 核酸疫苗 组织反应 

分 类 号：R383.24[医药卫生—医学寄生虫学]