水蛭热刺激分泌物与日本血吸虫成虫可溶性物质的免疫交叉反应性分析  

Analysis of immune cross-reaction between Whitmania pigra heat secretions and Schistosoma japonicum dissolvable substances

作　　者：黄进[1] 姜昌富[1] 董素娟[1] 魏兰英[1] Huang Jin;Jiang Chang-fu;Dong Su-juan;Wei Lan-ying(Department of Pathogenic Biology,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China)

机构地区：[1]华中科技大学同济医学院病原生物学系,武汉430030

出　　处：《中国血吸虫病防治杂志》2007年第1期28-31,58,共5页Chinese Journal of Schistosomiasis Control

摘　　要：目的分析水蛭热刺激分泌物(Wp—hs)与日本血吸虫成虫可溶性物质(Sj-Ds)间的共同组分,探讨两者之间免疫交叉反应的物质基础。方法通过升高温度刺激水蛭虫体产生Wp—hs,同时收集室温放置的水蛭分泌物作为对照,用十二烷基硫酸钠一聚丙烯酰胺凝胶电泳分析Wphs与Sj—Ds之间的共同蛋白组分;将这些共同蛋白组分分别与日本血吸虫病患者混合血清、兔抗Wp—hs免疫血清、健康人混合血清进行斑点酶联免疫吸附试验和免疫印迹试验,找出具有免疫交叉反应活性的共同蛋白组分。结果Wp—hs与Sj-Ds之间存在着分子量(12.6~85.4kDa)相近的蛋白条带。Wphs抗原在21.6~95.4kDa分子量范围内与日本l皿吸虫病患者混合血清反应尤为明显,且不与正常人血清反应;Sj-Ds(40.1~89.0kDa)与兔抗Wp—hs免疫血清能较好的结合。结论Wphs与Sj—Ds之间至少存在3种具有免疫活性的共同蛋白组分。Objective To analyze the mutual components between Whitmania pigra heat secretions(Wp-hs)and Schistosoma japonicum dissolvable substances(Sj-Ds),and explore the mechanism of the immune cross-reaction between them.Methods The heat secretions of Whitmania pigra obtained by stimulated with high temperature,and Sj-Ds were analyzed by SDS-PAGE technique,and used to react with the sera of patients with schistosomiasis,sera of healthy people,and sera of rabbits immunized with Wp-hs by Dot-ELISA and Western blot.The secretion of a Whitmania pigra at room temperature for 30 mins were collected as control.Results There were many similar molecular weight(12.6--85.4 kDa)protein bands between Wp hs and Sj-Ds,and some protein bands between 21.6-95.4 kDa from Wp-hs had intensive cross-reaction with the sera of pa tients with schistosomiasis,but no cross-reaction with the sera of healthy people.Some components of Sj-Ds whose molecular weight were from 40.1 kDa to 89.0 kDa could combine with the sera of rabbits immunized with Wp-hs.Conclusion There are at least three mutual immunocompetent protein bands with similar molecular weight between W.pigra and S.japonicum.

关 键 词：水蛭热刺激分泌物 急性期反应蛋白 日本血吸虫 免疫交叉反应 

分 类 号：R383.24[医药卫生—医学寄生虫学]