原花青素对SMMC-7721肿瘤细胞凋亡及其端粒酶活性的影响  

Effect of proanthocyanidins on apoptosis and telomerase activity in SMMC-7721 human hepatocellular carcinoma

作　　者：鲍永华[1] 郭永臣[2] 郭延君 吕学诜[4] 刘英芹[4] 赵志辉[1] 

机构地区：[1]吉林大学农学部畜牧兽医学院,吉林长春130062 [2]佳木斯大学附属第一医院核医学科,黑龙江佳木斯154002 [3]长春市九十中学西校,吉林长春130012 [4]佳木斯大学生物教研室,黑龙江佳木斯154002

出　　处：《西北农林科技大学学报（自然科学版）》2007年第1期37-40,共4页Journal of Northwest A&F University(Natural Science Edition)

摘　　要：采用M TT法、HE染色、DNA电泳、TUNEL法和TRAP法(端粒重复序列扩增法),研究原花青素对人肝癌细胞SMM C-7721株凋亡及端粒酶活性的影响,探讨了其抗癌机制。结果表明,原花青素可抑制SMM C-7721肿瘤细胞增殖,诱导细胞凋亡,降低细胞端粒酶活性。说明原花青素具有诱导肿瘤细胞凋亡和降低肿瘤细胞端粒酶活性2种抗肿瘤作用。To illustrate the effect of proanthocyanidins on apoptosis and telomerase activity in hepatocellular carcinoma as well as its antitumor mechanism,the effect of PC on SMMC-7721 cell line in vitro was studied by using MTT method,HE dying,DNA electrophoresis,TUNEL and TRAP （telomeric repeat amplification protocol）. PC inhibited the growth of SMMC-7721 cell line by inducing apoptosis and down-regulating the telomerase activity.

关 键 词：原花青素 肝癌细胞 细胞凋亡 端粒酶 

分 类 号：R73-361[医药卫生—肿瘤]