陕西辣椒病毒病的毒原鉴定及化学防治药剂筛选  

Virus identification and screening virusicides of pepper virus disease in Shaanxi Province

作　　者：陈丽[1] 樊民周 卫军锋 安德荣[1] 全鑫[1] 

机构地区：[1]西北农林科技大学植保学院与陕西省农业分子生物学重点实验室,陕西杨凌712100 [2]陕西省植保站,陕西西安710003

出　　处：《西北农林科技大学学报（自然科学版）》2007年第1期121-126,共6页Journal of Northwest A&F University(Natural Science Edition)

基　　金：西北农林科技大学创新团队资助项目

摘　　要：分别从陕西咸阳、兴平、杨凌、柔谷、眉县、岐山、凤翔等地采集辣椒病毒病标样126份,在室内通过单斑分离及回接验证得到5种分离物,采用鉴别寄主的生物学反应和DA S-EL ISA法鉴定,结果表明,引起陕西辣椒病毒病的毒原有黄瓜花叶病毒(CM V)、烟草花叶病毒(TM V)、烟草蚀纹病毒(TEV)、马铃薯Y病毒(PVY)和蚕豆萎蔫病毒(BBW V),其中CM V和TM V是优势毒原种群,分别占检测样品的60.31%和30.94%。在室内分别以CM V和TM V的枯斑寄主苋色藜和心叶烟为测试寄主,采用半叶法对接种叶片分别于接种前和接种后涂施病毒抑制剂,测试了7种病毒抑制剂的抑制效果。结果表明,接种前后涂施3.85%病毒必克水乳剂500倍液,均对黄瓜花叶病毒和烟草花叶病毒有很好的防治效果,且接种前涂施的防治效果较接种后涂施的防治效果好。126 typical samples of viral pathogens in pepper were collected from Xianyang,Xingping, Yangling, Rougu, Meixian, Qishan, Fengxiang and so on in Shaanxi province respectively. These samples were identified by biological reactions of different host plants and DAS-ELISA. The results showed that pepper in Shaanxi province were infected with TMV,CMV,TEV,PVY and BBWV,and the most predominant viruses were CMV and TMV,the proportion being 60.31% and 30.94% respectively. Inhibiting effect of several plant virus inhibitors against CMV and TMV were tested in laboratory. TMV was inoculated with Nicotiana glutinosa, CMV with Chenopodium arnaranticola, then these plant virus inhibitors were smeared over the half leaf before inoculation and 24 hours after inoculation. The results showed that the efficacy of 3.85% Bilken virusicide was the best to TMV and CMV when diluted 500 times either before or after inoculation. And the control effect before inoculation was better than that after inoculation.

关 键 词：辣椒病毒病 毒原鉴定 病毒抑制剂 防治效果 

分 类 号：S436.418.12[农业科学—农业昆虫与害虫防治]