大鼠心肌梗死后肿瘤坏死因子-α的早期表达与室性心律失常的关系研究  

Relationship Between Early Expression of Tumor Necrosis Factor-α and Ventricular Arrhythmias in Rats with Acute Myocardial Infarction

作　　者：肖华[1] 陈志坚[1] 廖玉华[1] 黄恺[1] 程翔[1] 王敏[1] 

机构地区：[1]华中科技大学同济医学院协和医院心内科,湖北武汉430022

出　　处：《医学临床研究》2007年第1期6-9,共4页Journal of Clinical Research

基　　金：国家自然科学基金资助项目(30400175)

摘　　要：【目的】通过研究大鼠急性心肌梗死(AMI)模型探讨心肌梗死后肿瘤坏死因子α(TNF-α)的早期表达情况及与心梗后室性心律失常的关系。【方法】结扎大鼠左前降支,建立AMI模型,设手术组(MI组)和假手术组(S组)。分10 min、20 min、30 min、60 min3、h、6 h1、2 h2、4 h共8个时间点记录大鼠心电图。用实时荧光定量PCR法和免疫组化法检测心肌梗死后各时间点TNF-α的mRNA和蛋白表达水平。组织病理检测炎性细胞浸润情况。【结果】与S组相比,MI组心肌组织中TNF-α从10 min起mRNA和蛋白表达水平均出现表达明显升高(P<0.05)。免疫组化分析定位染色显示,MI组TNF-α主要表达在梗死区存活的心肌细胞和非梗死区心肌细胞胞浆内。MI组心肌组织炎性细胞浸润在10 min2、0 min、30 min、60 min与S组相比无差异,从3 h起MI组炎性细胞表达增高(P<0.05)。MI组大鼠室性心律失常发生次数在各时间点均高于S组(P<0.05),并且与TNF-α分泌的时间窗重叠。【结论】TNF-α在心肌梗死后的早期就有大量表达,可能与心梗后室性心律失常发生有关。[Objeetive]To explore the relationship between early expression of tumor necrosis factor-α and ventricular arrhythmias in rats with acute myocardial infarction（AMI）. [Methods]Rats with AMI were induced by left anterior descending coronary branch ligation. The mRNA and protein expression levels of TNF-α were detected by real-time fluorescent quantitative PCR and histochemistry on 8 times： 10min, 20min, 30min, 60rain, 3h,6h,12h,24h after infarction. Electrocardiogram was recorded, inflammatory cells were detected by pathology. [Results]The mRNA and protein expression levels of TNF-α were higher in MI group than S group from 10min after infarction. TNF-α mainly expressed in the cardiocyte in infarction zone and cardiocyte endochylema in non-infarction zone. There were not differences in MI group and S group of the inflammatory cells at 10min, 20min. There were differences in MI group and S group of the inflammatory cells from 3h. The frequency of ventricular arrhythmias was higher in MI group than in S group（ P 〈0. 05）and the occurrence time of ventricular arrhythmias coincided with the secretion of TNF-α. [Conclusion]There is much early expression of tumor necrosis factor-α in rats with acute myocardial infarction and it may be related with the ventricular arrhythmias after AMI.

关 键 词：心肌梗塞 肿瘤坏死因子 心律失常 大鼠 

分 类 号：R542.22[医药卫生—心血管疾病]