A Novel Structure of LAPS Array for Cell-Based Biosensor  

作　　者：Ying Xu Hua Cai Qingjun Liu Lifeng Qin Lijiang Wang Ping Wang 

机构地区：[1]Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, China [2]State Key Lab of Transducer Technology, Chinese Academy of Sciences, Shanghai 200050, China

出　　处：《稀有金属材料与工程》2006年第A03期51-54,共4页Rare Metal Materials and Engineering

基　　金：This work was supported by the National Natural Science Foundation of China （Grant Nos. 30270387, No. 30570492）；the Project of State Key Laboratory of Transducer Technology of China （Grant No. SKT0403）；the Foundation for the Bureau of Zhejiang Province of China （Grant No. 20040197）.

摘　　要：The light-addressable potentiometric sensor (LAPS) is a semiconductor-based cellular biosensor with an electrolyte-insulator-semiconductor (EIS) structure.By depositing biocompatible layers on the sensing surface for cell culture, it can be used to detect bioelectrical parameters of cells.The characteristic curve for photocurrent versus applied bias voltage to the system shows a current-voltage curve (Ⅰ-Ⅴcurve).This technique can be used to detect the action potential changes towards different drugs based on the bias voltage dependence of an optical current,and provides a dynamic system by scanning light beam at the very cell on the sensor device.The LAPS overcomes the limitation of recording sites,but high spatial resolution and sensitivity are also paramount.This paper discussed a novel structure of LAPS array for extracellular monitoring to decrease potential noise level.Both characteristics of active recording array areas and cell culture conditions are measured.The light-addressable potentiometric sensor （LAPS） is a semiconductor-based cellular biosensor with an electrolyte-insulator-semiconductor （EIS） structure. By depositing biocompatible layers on the sensing surface for cell culture, it can be used to detect bioelectrical parameters of cells. The characteristic curve for photocurrent versus applied bias voltage to the system shows a current-voltage curve （Ⅰ-Ⅴ curve）. This technique can be used to detect the action potential changes towards different drugs based on the bias voltage dependence of an optical current, and provides a dynamic system by scanning light beam at the very cell on the sensor device. The LAPS overcomes the limitation of recording sites, but high spatial resolution and sensitivity are also paramount. This paper discussed a novel structure of LAPS array for extracellular monitoring to decrease potential noise level. Both characteristics of active recording array areas and cell culture conditions are measured.

关 键 词：light-addressable potentiometric sensor array LAPS sensitivity cell-based biosensor 

分 类 号：TG[金属学及工艺]