Study of Drift Behavior for RuO_x Sensing Membrane with Penicillinase Immobilized by GPTS  

作　　者：Jung-Chuan Chou Sheng-Hung Chen 

机构地区：[1]Graduate School of Electronic Engineering,National Yunlin University of Science and Technology,Douliou,Taiwan 640,China

出　　处：《稀有金属材料与工程》2006年第A03期228-229,共2页Rare Metal Materials and Engineering

摘　　要：The ruthenium oxide(RuO_x) thin film was prepared by using the R.F.sputtering system.The RuO_x pH-sensitive electrode possesses a nearly-Nernstian pH sensitivity(about 57 mV/pH).For the enzyme penicillinase was immobilized directly onto a RuO_x pH-sensitive surface by 3-glycidoxypropyltrimethoxysilane(GPTS).Some characteristics of the biosensor, such as sensitivity,linear range,and drift,are investigated and the difference between penicillinase immobilized and without are compared.According to the time-constant model,we can extract the time constant and amplitude at the response curve of the drift.Subsequently,the extracted parameters were used to simulate the mechanism of the drift behavior.The ruthenium oxide （RuOx） thin film was prepared by using the R.F. sputtering system. The RuOx pHosensitive electrode possesses a nearly-Nernstian pH sensitivity （about 57 mV/pH）. For the enzyme penicillinase was immobilized directly onto a RuOx pHosensitive surface by 3-oglycidoxypropyltrimethoxysilane （GPTS）. Some characteristics of the biosensor such as sensitivity, linear range, and drift, are investigated and the difference between penicillinase immobilized and without are compared. According to the time-constant model, we can extract the time constant and amplitude at the response curve of the drift. Subsequently, the extracted parameters were used to simulate the mechanism of the drift behavior.

关 键 词：ruthenium oxide pH-sensitive electrode PENICILLINASE drift behavior 

分 类 号：TB43[一般工业技术]