Study on Technique of Surface Plasmon Resonance Imaging Sensing for Biomolecular Interaction  

作　　者：Ding Xiang Rong Xiaofeng Deng Yan Yu Xinglong 

机构地区：[1]Dept. of Precision Instrument, Tsinghua University, Beijing 100084, China

出　　处：《稀有金属材料与工程》2006年第A03期483-487,共5页Rare Metal Materials and Engineering

摘　　要：High resolution of surface plasmon resonance (SPR) detection is of vital importance.SPR biosensing system resolution is determined by intrinsic sensitivity of biochip and light signal acquisition system.In this article,different signal acquisition system resolutions on photodetector were analyzed based on light intensity and phase detection.Result shows that charge coupled device (CCD) with larger numbers of pixels is potential to achieve higher detection resolution.A 64 pixel line array CCD and a 12 bit ADC can achieve resolution of 10^(-7) refractive index unit (RIU).In array detection mode,increasing of detection throughput is at the cost of decreasing system resolution.Simulation analysis indicates that,if noise is taken into account,phase modulation methods are capable of providing better noise reduction performance than intensity methods.High resolution of surface plasmon resonance （SPR） detection is of vital importance. SPR biosensing system resolution is determined by intrinsic sensitivity of biochip and light signal acquisition system, In this article, different signal acquisition system resolutions on photodetector were analyzed based on light intensity and phase detection. Result shows that charge coupled device （CCD） with larger numbers of pixels is potential to achieve higher detection resolution. A 64 pixel line array CCD and a 12 bit ADC can achieve resolution of 10-7 refractive index unit （RIU）. In array detection mode, increasing of detection throughput is at the cost of decreasing system resolution. Simulation analysis indicates that, if noise is taken into account, phase modulation methods are capable of providing better noise reduction performance than intensity methods.

关 键 词：SPR biosensing intensity detection phase detection RESOLUTION 

分 类 号：TG[金属学及工艺]