Monocrotophos Molecularly Imprinted Microspheres Prepared by Precipitation Polymerization in Acetonitrile  

作　　者：Shoulei Yan Zhixian Gao Yanjun Fang Yiyong Cheng 

机构地区：[1]Institute of Hygiene and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050, China [2]Department of Food Science, Huazhong Agricultural University, Wuhan 430070, China

出　　处：《稀有金属材料与工程》2006年第A03期621-622,共2页Rare Metal Materials and Engineering

基　　金：support by the National Natural Science Foundation of China(No.30371218);High Technology Research and Development Program of China(863 Project No.2004AA649110)

摘　　要：Molecularly imprinted microspheres (MIP) for monocrotophos have been prepared by precipitation polymerization in acetonitrile (ACN) 60℃,24 h,using methacrylic acid (MAA),ethylene glycol dimethacrylate (EGDMA) and 2, 2-azobisiobutyronitrile (AIBN) as functional monomer,cross-linker and initiator,respectively.The recognition mechanism was elucidated by UV-vis spectra and computer modeling.Equilibrium binding experiment was employed to investigate the rebinding properties,Scatchard analysis showed that specific binding sites formed in the imprinted microspheres,and there were two kinds of binding sites,one was high binding sites,the other was low binding sites.This microspheres can be useful affinity absorbent used for organophosphorus pesticides separation and purification in food and environmental analysis.Molecularly imprinted microspheres （MIP） for monocrotophos have been prepared by precipitation polymerization in acetonitrile （ACN） 60℃, 24 h, using methacrylic acid （MAA）, ethylene glycol dimethacrylate （EGDMA） and 2, 2-azobisiobutyronitrile （AIBN） as functional monomer, cross-linker and initiator, respectively. The recognition mechanism was elucidated by UV-vis spectra and computer modeling. Equilibrium binding experiment was employed to investigate the rebinding properties, Scatchard analysis showed that specific binding sites formed in the imprinted microspheres, and there were two kinds of binding sites, one was high binding sites, the other was low binding sites. This microspheres can be useful affinity absorbent used for organophosphorus pesticides separation and purification in food and environmental analysis.

关 键 词：MONOCROTOPHOS molecular imprinting microspheres precipitation polymerization 

分 类 号：TG[金属学及工艺]