枇杷β-胡萝卜素羟化酶基因片段的克隆和序列分析  

Molecular cloning and sequence analysis of β-carotene hydroxylase gene fragment from loquat

作　　者：刘兴满[1] 陶俊[1] 梁国华[1] 

机构地区：[1]扬州大学园艺与植物保护学院,江苏扬州225009

出　　处：《扬州大学学报（农业与生命科学版）》2006年第4期101-103,共3页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：江苏省自然科学基金(BK2004056);江苏省高校自然科学基金(03KJB210154)

摘　　要：根据蔷薇科植物β-胡萝卜素羟化酶基因的保守区序列,设计1对PCR引物,以枇杷基因组DNA为模板,采用PCR方法扩增出长约450 bp的DNA片段,克隆人pMD-18T载体中,测得该基因片段长为440 bp,编码145个氨基酸,属于开放阅读框的一部分,不含内含子。在GenBank中进行同源性检索,结果该序列与苹果β-胡萝卜素羟化酶基因同源性为95%,证明它属于枇杷的β-胡萝卜素羟化酶基因。A pair of primer, designed from conserved regions of β-carotene hydroxylase from rosaceae, was used to amplify homogeneous gene by polymerase chain reaction （PCR）. As a result, one about 450 bp fragment was obtained, and then cloned into pMD-18T vector and sequenced. The result suggested that the 440 bp belong to a part of ORF （open reading frame） and encode 145 amino acids. It is suggested that the sequence is 95% identical to the β-carotene hydroxylase gene from apple, it could be deduced as β-carotene hydroxylase gene fragment of loquat.

关 键 词：枇杷 Β-胡萝卜素羟化酶 基因 克隆 

分 类 号：S667.3[农业科学—果树学]